FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_negative_control_te_pcr2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_negative_control_te_pcr2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length150
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACTGGGCCTTATCAACAGATGGGGTTCCGTGGGGAAAAAAGAGGCTATG11.7241379310344827No Hit
ATCCAAGTTCTGGTAGTATGGGCTCCCGCGGCGAGTGATCTCTGCTGCCA11.7241379310344827No Hit
ACTACCAGCACTGCCATTGATGTGCTCATGATGATCTTTGTGGTCATTCT11.7241379310344827No Hit
GAACAAGAGAGCAAATAAGTGAGGTCAATGGTGACAGCCTGTTCAAACGA11.7241379310344827No Hit
CATAAGGAGGAGGCGTCTATTGGACCAGACGATAGTCGTGGATATGTGAA11.7241379310344827No Hit
CAGTAGTCCTACCACATTAATAGGGTCTACTACCCTCACAGCTGTCAATA11.7241379310344827No Hit
CCTTGTGAGTAACAGTAGTCCTACCACATTAATAGGGTCTACTACCCTCA11.7241379310344827No Hit
GTAAATTACATTTAGGATCAACCAGCATACCAACAGCACGGCACTCACCC11.7241379310344827No Hit
CCAGGATGGCCTCATAGCCTCGCTCTATATGCCTGAGGCCGATAAGGTAA11.7241379310344827No Hit
GCTCCGATGTCCCCGTCCTTTGTCTTGAATATTCCAGGCAGGGTCTGAAT11.7241379310344827No Hit
AGATAAGGCCCAGGAAAGAACCAGAGAGCAACTTAGTGAGGTCAATGGTG11.7241379310344827No Hit
CTCTTGGAGTGCTTGTGATTCTACTCATGGTGCAGGAGCTGTCTCATATA11.7241379310344827No Hit
CATTAATAGGGTCTACTACCCTCACAGCTGTCAATCCCAGGGCCATGACA11.7241379310344827No Hit
GAACAGTGAAGAGCTTGAAAACCGATTTAAGGAATGTCCAGGCTCCAAGG11.7241379310344827No Hit
AGTAAAAAGAGAAAACTCAAGGGGTTCTTCTTCAACTAGCCGGTCATCCG11.7241379310344827No Hit
AGAGGATACATATCAACAAGGGTTGAAATGGGAGAGGCGGCTACCATTAT11.7241379310344827No Hit
AACACGGGGTTGGCGGTTATCATCCTTCAAACACGGGTCAGGGTCTCCAT11.7241379310344827No Hit
GTTGTCAATGGCTTCCTGAAACCTCTCTGTCATGTGTCCTGGCAGTGTTC11.7241379310344827No Hit
GTGTTGAAGTCATAAAAATGGCATCCGCCTCGCCCATTTCAACCATTGTT11.7241379310344827No Hit
GTAGCTCACTTGGCATTTGTAGCGGCATTTAAAGTCAGACCAGCCTTGCT11.7241379310344827No Hit
TCATGATTTGGCATTCCAACCTGAATGATGCCACATATCAGAGAACAAGA11.7241379310344827No Hit
GCATTATTGCTTCAAGCCAGTGTGAATGGACTACATCAGTCTCTGAACAC11.7241379310344827No Hit
GTTGAAGAAGAGAATGACCACAAAGATCATCATGAGCACATCAATGCAGT11.7241379310344827No Hit
ATCAGGTGCATTGGAGTCAGCAATAGAGACTTCGTGGAGGGCATGTCAGG11.7241379310344827No Hit
GTCCATGATCTGTACGTGGCACTTGTTCACTCCCAATGTGGTAGCAAACG11.7241379310344827No Hit
GAGCAAATATCAGGCCAACGGCTGAGAGAACTTCACTAGGGGGCCAGCTC11.7241379310344827No Hit
TTTCTGGGCAGCACCAGCAAAAAACAATAATACCCATGGGGAACAAAACA11.7241379310344827No Hit
ACTCTTTCCCGAGACCACATAGCTGGCAATTCGCAAGCCTACTGCAGCCA11.7241379310344827No Hit
CTCCAGGCTTCAACATCATTATAGATGAATACCCCCGTGCCACATCTCGT11.7241379310344827No Hit
GTCTTTAGCTGGTCCACTCAGCCACAACAACACCAGAGAGGGTTACAGAA11.7241379310344827No Hit
TCTGTGGAATGAACCCAATCGCTATACCTTTTGCTGCAGGAGCGTGGTAT11.7241379310344827No Hit
ATCCGGCGGTTAGGCGAGAGGGAAAGTGCTCCTGGGGCGATGGGGCCGTG11.7241379310344827No Hit
GTGTTGCACCAGCAATCGACATCATCTGGTTCCACTCCCTCATCCAGCAT11.7241379310344827No Hit
CTATTGCTGACTCCAATGCACCTGATACTGTATGCCGGGGCAATCAGCAG11.7241379310344827No Hit
CACTGAATGAATGTCAGCTTGAGCAAAGACCATGGAAATGATTACTTGTG11.7241379310344827No Hit
CAGAATGAACACTGGAGGAGATGTAGCTCACTTGGCATTGGTAGCGGCAT11.7241379310344827No Hit
CTCTGGTGTTGTGGTGGCGGAGTGGACCAGCTAAAGACAGGGGTATGATA11.7241379310344827No Hit
GTGGAGAGGTCCACAAAGATTGCCAGTGCCTGTGAATGAGCTGCCCCATG11.7241379310344827No Hit
CACTCTCATGGGTGGCCCATAATCCTCTACCAAGGAGAAATCACAACTCT11.7241379310344827No Hit
CTTTTTGGCTCGTCGAGCTTCCCAAAAGCCAGGCAATGGCAACGGCCACT11.7241379310344827No Hit
CACTAAAACATGGAATAAGCCCACTTAACATACCCCAAAATTATATATTC11.7241379310344827No Hit
GGTTCCTGGTGGTGTGGCAGTCATAAAAATGGCAGCCGCCTCGCCCATTT11.7241379310344827No Hit
AATGAGAACTATTGGGACTCATCCTAGCTCCAGTGCTGGTCTGAAAGATG11.7241379310344827No Hit
GGGTATGATAAGATCACTATCTTCTACTCCATCTGTCAACAATGTGTGAG11.7241379310344827No Hit
GTGGTGGCTGAGTGGACCAGCTAAAGACTTGGGTATGATAAGATCACTTT11.7241379310344827No Hit
ATGGGAAGGAGAGAAGAAGGAGAAACAATGGGATTCTCAATGGACATTGA11.7241379310344827No Hit
TAATGGACCACTACTCACGGGGGGTGCTTGGGATTGTACGAATGGTGCTG11.7241379310344827No Hit
ATACAGCTCAGCAGGAAGACTTTTGAGACAGAATTTCAGAAAACAAAAAA11.7241379310344827No Hit
GTGGACAGAGGTTGGGGAAACGGTTGTGGACTTTTTGGCAAAGGGAGCTT11.7241379310344827No Hit
TGCCAAGTTAGATACATATGATCCAGTGTTAATTTCTGAGAAAGTAGCAC11.7241379310344827No Hit
GCCATTGAATGAACCAGAGAGCAACATAGTGAGGTCAATGGGGACAGCGG11.7241379310344827No Hit
TACCTCGGCCATGTTACTAACCGTCGTCGTGACCAACTCTATGTCAACTG11.7241379310344827No Hit
TGTGGCGCCTAAGTGTGTGAATGTGGGCAGTTTCATTGGATGAGCGCAGA11.7241379310344827No Hit
AATGGTGACAGCGGGGTCAACCGATCATATGGACCACTTCTCTCTTGGAG11.7241379310344827No Hit
GGTTGCTTCCGCTCTTGGTGAATTAGGCGTAACCTCGACTTTCGCTCTAT11.7241379310344827No Hit
TCTTTGTGGTCATTCTCTTCTTCAACCCCTCCTGCACCATGAGTAGAATC11.7241379310344827No Hit
CCACCAGGAACCCGTGATGCGTTTCCTGACTCTAACTCACCAATCATGGA11.7241379310344827No Hit
ATATGACACGCCCTTCAATCTAAGCTTGTCCATTTTTAGGCGGCATTTCA11.7241379310344827No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGTG50.0144.08
CACTCTC50.0144.01
TCATGGG50.0144.06
TGGGTGG50.0144.09
CATGGGT50.0144.07
CTCATGG50.0144.05
TCTCATG50.0144.04
CTCTCAT100.072.03
ACTCTCA100.072.02
Produced by FastQC (version 0.11.2)