FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_negative_control_te_pcr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_negative_control_te_pcr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences101
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65.9405940594059405No Hit
GTTGCTCTCTGGTTCTTTCCTGGGCCTTATCTCCATTCCATACCAGCAGC10.9900990099009901No Hit
TTAATACATTGTCATAGGAGTTGGGGACAAGAAACTCACCCACCACTGGA10.9900990099009901No Hit
CTGCTAGTGTGGTTAGGTTTGAACACAAAGAATGGATCTATCTCCCTCAC10.9900990099009901No Hit
GGGTAAGGGCATTCACCAGATTTTTGGAGCAGCCTTCAAATCACTGTTTG10.9900990099009901No Hit
CCCTGCATACTGCACCTCCACTGTGACTGTTCCATGCAGTGTTTCAGCTG10.9900990099009901No Hit
TTATAGATGAATACCCCCGTGCCACATCTCGTTTCATTTTTTGAGAAGTC10.9900990099009901No Hit
TGATCGGTTGACCCCGCTGTCACCATTGACCTCAATAAGTTGCTCTCTGG10.9900990099009901No Hit
CACTAGCAGCGTGCCTATGAGGATCTGTGAGAACCAGGACCTGTCTCTTA10.9900990099009901No Hit
GGGTATGATAAGATCACTTTCTTCTACTCCAAATGTCCACAATCTGTCAC10.9900990099009901No Hit
GGCTATGAAACTGACGAAAATAGAGCCAAAGTCGAGGTTACTCCTAATTC10.9900990099009901No Hit
CCAAGTATATGACTTTTTGGCTCGTCGAGCTTCCCAAAAGCCAGGAAATG10.9900990099009901No Hit
TAATAGCTCCAGCCCCCTCTGCCACATCCGAGGTCAACAACCATCCCATA10.9900990099009901No Hit
TACCGGTCCCTCCAGGCTTCAACATCATTATAGATGAATACCCCCGTGCC10.9900990099009901No Hit
GCATTGGTAGAATTCAAGGATGCCCACGCCAAGAGGCAAACCGTCGTCGT10.9900990099009901No Hit
CTTGAAATGCCGCCTAAAAATGGACAAGCTTAGATTGAAGGGCGTGTCAT10.9900990099009901No Hit
CTTCCACACCAGTGTCTGGCTTAAGGTCAGAGAAGATTACTCATTAGAAT10.9900990099009901No Hit
ACCTGATACTGTATGCCGGGGCAATCAGCAGTATCATGACCAAGTATATG10.9900990099009901No Hit
GTAGTCTTCTCTGACCTTAAGCCAGACACTGGTGTGGAAGAATACAAAAA10.9900990099009901No Hit
TCCCCCAGGACTGCCATTCTCTTGGCGCCTCTCACAGTGGCCTCAAATGC10.9900990099009901No Hit
GTGCTGCTTGCCACGCGATTCAGTGTAAGGTGGAATGGAGTACGATATAG10.9900990099009901No Hit
ATCTCCCTCACATGCTTGGCCCTGGGGGGAGTGATGATCTTCCTCTCCAC10.9900990099009901No Hit
ATTCTTGAACAGGGATTTGAAACATATGATGTACAATAATCCAAAAACAC10.9900990099009901No Hit
ACATGTACTTGGATAGGAGCGATGCCGGGAATGCCATTTCGTTTGCTACA10.9900990099009901No Hit
GGGGTATTCATCTATAATGATGTTGAAGCCTGGAGGGACCGGTACAAGTA10.9900990099009901No Hit
GCACTGCGGCATTCACATTCACCAAGGTCCCAGCTGAAACACTGCATGGA10.9900990099009901No Hit
GACCTGGGTTGATGTTGTCTTGGAACATGGAGGCTGCGTTACCGTGATGG10.9900990099009901No Hit
AGTGCATACTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTC10.9900990099009901No Hit
CATCTGTCCACAATGTGTGAGAATTTGGCCATTCACATGTTTTCATCTCA10.9900990099009901No Hit
ACATACCATTGCATTGGCATGCTGGGGCATACACCGGAACTCCACACTGG10.9900990099009901No Hit
GATCATGATGATCTTATTGGTCATTATCAACTTAAACCACTACTGCACAA10.9900990099009901No Hit
ATCATACCCAAGTCTTTAGCTGGTCCACTCAGCCACCACAACACCAGAGA10.9900990099009901No Hit
GCACCAGCAATCGACATCATCTGGTTCCACTCCATCATCCAGCATAGGGC10.9900990099009901No Hit
AATCAGGTGGGCCCTTTTCAGCATACATGTGTCATTCTTTTCACTTTCAA10.9900990099009901No Hit
CCATATCTGAGATCAACTAATGCAAGTGGAAGTGTCATTGAGGAATCGTG10.9900990099009901No Hit
GAAAAACCCCGTGCCACATCTAGATTCCTATTTTGACAAGACCACTGAGC10.9900990099009901No Hit
CTTATACACATCTGACGCTGCCGACGACGGAGAGAGTGTAGATCTCGGTG10.9900990099009901No Hit
ATACTGTATGCCGGGGCAATCAGCAGTATCATGACCAAGTATATGACTTT10.9900990099009901No Hit
GTCCACTAAAGTTCATTTGCAGACATATTGAGTGTCTGATTTCTTGTAAA10.9900990099009901No Hit
GGTTTCATGACATCCCATTGCCTTGGCATGCTGGGGCAGACACAGGAACT10.9900990099009901No Hit
AGAGGATGAGATGGATGGTTCAAAGGGAAAGCTGTTCTCTGGCCACTTGA10.9900990099009901No Hit
CACTAGCAGCGTGCCTATGAGGATCGGTGAGAACCAGGACATTCCTCCAA10.9900990099009901No Hit
CCTTTCCAATGGTGCTACCACTCCTATGACAGTGGTGGGTGATTTTCTTG10.9900990099009901No Hit
ACAGGAGATTCCTCCAAACAGTGATTTGAATGATGATACATAAATCTGGT10.9900990099009901No Hit
TTATAGATGAATACCCCCGTGCCACATCTCGTTTCCTTTTTTGAGAAGTC10.9900990099009901No Hit
CCCGGGGTTCCTGAATATCCAGTTTTCAACCTTGATCAATTGCTTCGTGT10.9900990099009901No Hit
AGATCACTGGGCGCTGCCTCCCTACCGTTAACAGCTGTTACTATGACGGC10.9900990099009901No Hit
GGGGGGGGGGCGGGGGGGGGGGGGCGGGGGGGGGGGGGGGGGGGGGTGGG10.9900990099009901No Hit
GCAATCTAACAGTGAAGATCATTAAAAACAGTTTGAAGAATGTCATGAAA10.9900990099009901No Hit
ATTCATCTATAATGATGTTGAAGCCTGGAGGGACCGGTACAAGTACCATC10.9900990099009901No Hit
GTGTACTCTTCTCTGACGTTAAGCCAGACACAGGTGTTGAAGACTACAAA10.9900990099009901No Hit
ATCACGGGGTTGGAGGTTATCAGCCTTCCAACTGGGGTCAGGGATTTCTC10.9900990099009901No Hit
AGCTTAGGACTTGACTGTGAACCAAGGACAGGCCTTGACTTTTCAGATCT10.9900990099009901No Hit
TACCAGCACAGCCATTGATGTGCTCATGATGATCTTTGTGGTCATTCTCT10.9900990099009901No Hit
TCACTGTGAGCGGCCTCCCTTCACTTAACAGATGATACTATGACGGCTGG10.9900990099009901No Hit
GGCTTTCCAGCCATGGGGCAGCTCATTCACAGGCACTGGCAATCTTTGTG10.9900990099009901No Hit
ACCAAGGTCCCAGCTGAAACAATGCATGGAACAGTCACAGTGGAGGTGCA10.9900990099009901No Hit
GCAAGGCTGGTCTCCTTCATTTTCAGAGCCAATTGGCCCTGTCTCTTATA10.9900990099009901No Hit
GATCCCCTCTTCCCAGGCCTGCTTGACTGCTGCTCCCAATCTGCGGGGGG10.9900990099009901No Hit
CCCATCAGGATCACAAGCTTGGCCAGGTCACTCATTGAAAATCCTCCCAA10.9900990099009901No Hit
AATGGAGGGTCAAGCTCCGACAGCATCTTTGAGTTCTACGTGCTTTCAGT10.9900990099009901No Hit
GTGATCTGGGCTATTGGATTGAAAGTGAAAAGAATGACACATGGAGGCTG10.9900990099009901No Hit
GAACCAGATGATGTCGATTGCTGGTGCAACACGACATCAACTTGGGTTGT10.9900990099009901No Hit
TGAGCACAGAGCATGGAATAGTTTTCTTGTGGAGGATCCCGGGTTTGGAG10.9900990099009901No Hit
AGCTTAGATTGAAGGGCGTGTCATATTCCTTGTGCACTGCGGCATTCACA10.9900990099009901No Hit
CAATGATACAGGATATGAAACTGACGAAAATAGAGCGATAGTCGAGGTTA10.9900990099009901No Hit
GGCCATGGCACAGTGAAGAGCTTGAAATCCGGTTTGAGGAATGTCCAGGC10.9900990099009901No Hit
TTCTAATGAAATCCAAATGATGTTTACAAATAATGAAAAGTATGAGATAC10.9900990099009901No Hit
GCACTGATAGCATTATCCGATACTCCAGATTTTCCGGTTGAATGCTCTTC10.9900990099009901No Hit
TCACTATTTCTGACTCCAATGCACCTGATACAGTATGCCGGCGCAATCAC10.9900990099009901No Hit
AGTTCATCTTGGTGGCGGCAAGGGACCACAATGGATCTCCCATCCTTGAG10.9900990099009901No Hit
TTCTTACATTGTCATAGGAGTTGGGGACAAGAAAATCACCCACCACTGGC10.9900990099009901No Hit
GTACGTGGCACTTGTTCACTCCCAATGTGGTAGCAAACGAAATGGCCTTC10.9900990099009901No Hit
GGCCTTGACTTTTCAGATCTGTATTACCAGACCATGAACAATAAGAATAG10.9900990099009901No Hit
AGCTCTTCACTGTGCCATGGCCCTTTCACTTGGGTTCTGTAACCCTCTCT10.9900990099009901No Hit
ATTATAGATGAATACCCCCGTGCCACATCTCGTTTCCTTTTTTGAGAAGT10.9900990099009901No Hit
CCTCCATGTGTCATTCTTTTCACTTTCAATCCAATAGCCCAGATCACTGT10.9900990099009901No Hit
CTGAAAGCACTGAGAACTCAAAGATGATGTTGGAGCTTGACCCACCATTT10.9900990099009901No Hit
GGTATGGAATGGAGATAAGGCCCAGGAAAGAACCAGAGAGCAACTTAGTG10.9900990099009901No Hit
GCATCATCCACATCGACTGAGGCGGTTCCATGCAGTGTTTCCGCTGGGAC10.9900990099009901No Hit
GCATTGAGCTCCCCTTCTACTGATTTCCACATGATGTTTTCCATTCTTGA10.9900990099009901No Hit
CCAGCAGCCGTCTTTTGCTCGAAACGATAGTGGGGGCATTGTGCATTCCC10.9900990099009901No Hit
ATATGACACGCCCTTAAATCTAAGCTTGTCAAGATTTAGTCGGCATTTCA10.9900990099009901No Hit
AGAGATCTCTGCTGCCATGTCTGTAGTCAGCAGGAGGCCAATGATTACGA10.9900990099009901No Hit
GTTCATGGTAAGGTAATACAGATCTTAAAAGTCAACGCCTGTCCTTGGTT10.9900990099009901No Hit
GATCTGTGAGAACCAGGACATTCCTCCAAACAGTGATTTGAAGGCTGCTC10.9900990099009901No Hit
GCATAGGAGTGGTAGCACCATCGGAAAGGCATTTGAGGCCACTGTGAGAG10.9900990099009901No Hit
TCCCACAACAACTGTCAGTTGAACTCCATTCTCCTCTAGGCTGTCTGTTA10.9900990099009901No Hit
TCACACGGCTCTCGCTGGAGCTATAGAGGATGAGATGGATGGTGCAAAGG10.9900990099009901No Hit
CTACCGCTCTTGGTGAATTAGGAGTAACCTCGACTTACGCTCTAATTACG10.9900990099009901No Hit
AACCCCGGGTTTGCGCTAGTGGCCGTTGCCATTGCCTGGCTTTTGGGAAG10.9900990099009901No Hit
CATTAGTGGACAGAGGTTGGGGAAACGGTTGTGGACTTTTTGGCAAAGGG10.9900990099009901No Hit
GTTTTCTTGTGGAGGATCACGGGTTTGGAGTCTTCCACACCAGTGTCTCT10.9900990099009901No Hit
AGTTGGAATTAAGAAATTTCATGGTTGTAAATGTCTGAGATGTCAACATA10.9900990099009901No Hit
GGATGAGGGAGAGGAACCAGATGATGTCGATTGCTGGTGCAACACGACAT10.9900990099009901No Hit
ATTCTAACCAGGTCTGCGACCGCGTTTGCAACTTCCTTGTAGAGTGAGAA10.9900990099009901No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers