FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_mosquito_9_pcr2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_mosquito_9_pcr2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences343284
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGAGATGGTGAGAGGCAGGAGTTACTCTAATGAACATATGTGTCC37971.1060812621619418No Hit
GCACTGGAGATGGAGCGTGCACCACTTGGCTCTGCTCTGCTTCTTATGAA28480.8296337726197551No Hit
GCACTGGAGATGGCTGGCACAGGCCATCAGACTCCCTAGAAAGCAGCAGT26200.7632164621712635No Hit
GCACTGGAGATGGGAAACAAGGACAGATATAAATACAGAAAGCATCTATT21520.6268861933559385No Hit
GCACTGGAGATGGCTACTGCTATGAGTAACACTTGTCCCATCTTCTTCTC21250.6190209855396698No Hit
GCACTGGAGATGGACCTGGCCCGCCTTTTGCTGCCACACAGCTTGATGGT13440.3915125668542664No Hit
GCACTGGAGATGGCGGTCAGGGACAGCCAGCCTTTTACCCTAGATGGTTC12870.3749082392421435No Hit
GCACTGGAGATGGAGATGCCGAGGACGAATGAGTTGCAGATGCAGTTGGG12680.3693734633714359No Hit
GGAGATGGAGCGTGCACCACTTGGCTCTGCTCTGCTTCTTATGAAGACAT8380.24411274629752622No Hit
GCACTGGAGATGGAGGGCTAAAGGGTAGGGGATGGGCTTCTTAGCCACAG7210.21003017909369504No Hit
GCACTGGAGATGGGAAGCAAGACCCGGCTTCGTGTGGAAAGACCAGCTTC6750.19663019540671867No Hit
GATATAAATACAGAAAGCATCTATTCTGTTTCATGAAAGTGAGATGAATG4640.13516505284254438No Hit
GGTCTGAACAGACCCTAGCATCCATCCATCTCGGTTTGAGCACTCTCTTC4490.1307954929446173No Hit
TCCCATGACAGCCCGCATGGGGACAGAGTAGGTTTGCTGGCTGCTTAGGG3840.11186073338693328No Hit
GAGATGGAGCGTGCACCACTTGGCTCTGCTCTGCTTCTTATGAAGACATG3830.11156942939373814No Hit
TACTACAACCCATCAGAGTCCCTAATTACAATCTCTACATCATGGATGAA3820.111278125400543No Hit
GGAGATGGAGATGCCGAGGACGAATGAGTTGCAGATGCAGTTGGGCCTGA3760.10953030144137217No Hit
GTCTACGACAATCCTTAGAGGGTACTCCCTGGAAAAGTACAGTGTATCCT3760.10953030144137217No Hit
TCCTTAGAGGGTACTCCCTGGAAAAGTACAGTGTATCCTAAAAATATAAA3640.1060346535230305No Hit
CTATTAATGTGGTAGGACTACTGTTACTCACAAGGAGTGGGAAGCGGAGC3520.10253900560468882No Hit
TTTCTAACCTACACCTCATGAGGCTCTGGTGAGAGAAGGAATAAGGAGCA3480.10137378963190828No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGAG38950.0114.951543
GATGGCG3800.0109.853279
GCACTGG41600.0107.4558641
TGGAGAT41950.0106.7309345
CACTGGA43600.0102.36162
CTGGAGA46850.096.182624
GGAGATG48900.092.886456
GAGATGG51400.086.12797
GATGGGA7400.083.6441049
AGATAAG2850.080.823371
AGATGGG13400.071.435728
GATGGCT13700.055.6870089
AGATGGT15900.054.3191228
AGATGGA24400.054.2745938
TAAGGCC5500.047.1163524
GATGGAG18800.046.3229459
GATGGAC6700.045.11739
AGATGGC33350.039.0617188
GTATAAG750.001535037838.3911061
GATGGTG20600.037.034569
Produced by FastQC (version 0.11.2)