FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_mosquito_4_-_101817_pcr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_mosquito_4_-_101817_pcr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences682334
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAGTGCGTGGCCGAGGTTGGGCGGCGGTGCCTAGCCAAGTTCGCGGAA41620.6099652076549021No Hit
GTGCGTGGCCGAGCAGGTGCGCGAGGTGGAGGAGTGCCTGGCCTTCTGCG39410.57757637755117No Hit
GTCAGTGCGTGGCCGAGCAGGTGCGCGAGGTGGAGGAGTGCCTGGCCTTC34760.5094279341202402No Hit
GTGCGTGGCCGAGGTTGGGCGGCGGTGCCTAGCCAAGTTCGCGGAACAGC31600.4631163037456729No Hit
GTCTTAACATAGGCAACATGAGCTCTAAGCTGAGCAGATGCAGTATCGCG19260.2822664560171412No Hit
GTGCGTGGCGGTTCTGATTTCGGTGAATCGCGCGACAACCGTGTTGCGAG17370.25456741126779553No Hit
GTGCGTGGCGGCTGGTAGGAAGTAGTAAGTAAGAAAAGAAGAATACAAGT12940.18964319526800658No Hit
GTATTACCTGACCATGAACAATAAGCATTGGTTGGTGCACAAAGAGTGGT12900.18905697209870825No Hit
CCTTAAACCTGTCTTGTAACCTTGATACTTACTTTCAGCCTCCATGTGTC12740.18671207942151496No Hit
GGTAATACAGATCTGAAAAGTCAAGGCCTGTCCTTGGTTCACAGTCAAGT12550.18392751936734797No Hit
GTCAGTGCGTGGCTCCAGGTGCGGATGTCTGAAGGACCACTCAGGAACCT11720.17176338860440782No Hit
ATCTAAGCTTGTCCATTTTTAGGCGGCATTTCAAGTGGCCAGAGAACAGC11660.17088405385046035No Hit
ATGCTGGATGAGGGAGTGGAACCAGATGATGTCGATTGCTGGTGCAACAC11320.16590115691142462No Hit
GGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCT11220.16443559898817883No Hit
TCCCAGGCGCGTGGCGTCGTCCGCCGTGGCCAGGTAGGGGTCGTCACCGT11060.16209070631098552No Hit
GTCATAGGAGTTGGGGACAAGAAAATCACCCACCACTGGCATAGGAGTGG10470.1534439145638353No Hit
GTGCGTGGCTCCAGGTGCGGATGTCTGAAGGACCACTCAGGAACCTTAGT10380.1521249124329141No Hit
GTCAGTGCGTGGCGGTTCTGATTTCGGTGAATCGCGCGACAACCGTGTTG10290.15080591030199286No Hit
GGCCGAGGTTGGGCGGCGGTGCCTAGCCAAGTTCGCGGAACAGCCGGGTC10290.15080591030199286No Hit
GTGCGTGGCAAACTAGATGTTGGTAACACGAACAATAGCGACATTAGCAT9860.14450401123203593No Hit
GTGGAGGAGTGCCTGGCCTTCTGCGGCCAGCGGCCGGTGCGCTGGCTGCT9840.14421089964738676No Hit
CTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTA9680.14186600697019347No Hit
AACTAGATGTTGGTAACACGAACAATAGCGACATTAGCATTCTTAGGTTA9290.13615033106953486No Hit
GTCAGTGCGTGGCGGCTGGTAGGAAGTAGTAAGTAAGAAAAGAAGAATAC9220.1351244405232628No Hit
GTATATGACTTTTTGGCTCGTCGAGCTTCCCAAAAGCCAGGCAATGGCAA9200.13483132893861363No Hit
TTCATATCCTGTATCATTGACAATCATCCCGCTATGCTGGGAGCCATGCA9040.13248643626142037No Hit
AGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTAATTTGGATTTG8950.13116743413049914No Hit
TCTTAGAACACGTAAACTTGGCACATGTCACCAAGCTCCCTTTGCCAAAA8340.12222753079869976No Hit
TAATAAAGAAGTTCAAGAAAGATCTTGCTGCCATGTTGAGAATAATCAAT8330.12208097500637517No Hit
TCCTTGTAGAGTGAGAAGGGAGCGTCACGGCTCTTCTAGATCGCCGTGCC8270.12120164025242769No Hit
GCCGAGCAGGTGCGCGAGGTGGAGGAGTGCCTGGCCTTCTGCGGCCAGCG8250.12090852866777854No Hit
GTCTTTAGCTGGTCCACTCAGCCACCACAACACCAGAGAGGGTTACAGAA8250.12090852866777854No Hit
TCCTAGAGGAGAATGGAGTTCAACTGACAGTTGTTGTGGGATCTGTAAAA8210.1203223054984802No Hit
GAATATGACACGCCCTTCAATCTAAGCTTGTCCATTTTTAGGCGGCATTT8170.1197360823291819No Hit
GGTAGGGGTCGTCACCGTCGAGCACCACCAGGTCGGCACGCAGGCCCGGG7960.11665841069036571No Hit
GTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTAATTTGG7940.11636529910571657No Hit
TCCGTACACAACCCAAGTTGATGTCGTGTTGCACCAGCAATCGACATCAT7840.11489974118247075No Hit
GAGTTATGAGTGCCCTATGCTGGATGAGGGAGTGGAACCAGATGATGTCG7820.1146066295978216No Hit
AGGTTGGGCGGCGGTGCCTAGCCAAGTTCGCGGAACAGCCGGGTCAGCGC7790.11416696222084786No Hit
CTCAATATGTCTGCAAAAGAACATTAGTGGACAGAGGTTGGGGAAACGGT7760.11372729484387412No Hit
GTTAGTAACATGGCCGAGGTAAGATCCTATTGCTACGAGGCATCGATATC7650.11211518112830374No Hit
GTAGTATGCACTCCCGCGTCTAGTGATCTCTGCTGCCATGGCTGTAGTCA7630.11182206954365456No Hit
GTGCGTGGCACGGCCATTGCTCGAATTGCCAACCAGGCCAAAGCAAATCC7590.11123584637435625No Hit
CTCCAAGAGAGAAGTGGTCCATATGATCGGTTGACCCCGCTGTCACCATT7520.1102099558280842No Hit
TTAGTGGACAGAGGTTGGGGAAACGGTTGTGGACTTTTTGGCAAAGGGAG7480.10962373265878586No Hit
GGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAG7430.10889095369716298No Hit
GTAGTAAGTAAGAAAAGAAGAATACAAGTAGAAGTTTAAGAGAAAAGGTA7340.10757195156624175No Hit
CTTCTAGATCGCCGTGCCTCACCTTTTTTGTGATGACAGGTTCCGTACAC7310.10713228418926801No Hit
AGCTTAGATTGAAGGGCGTGTCATATTCCTTGTGCACTGCGGCATTCACA7240.10610639364299596No Hit
AGGTGGAGGAGTGCCTGGCCTTCTGCGGCCAGCGGCCGGTGCGCTGGCTG7150.10478739151207474No Hit
GTACAAGTACCATCCTGACTCCCCCCGCAGATTGGCAGCAGCAGTCAAGC7090.10390805675812725No Hit
ACTCTATGTCAACTGTTGGCTTGTCCTGTGCCATCACGGTAACGCAGCCT6970.10214938725023229No Hit
TCCATGCTCTGTGCTCAAGCGGACATTCCTTCAGTGTGTCACCGTCGACA6920.10141660828860939No Hit
TTATAGATGAATACCCCCGTGCCACATCTCGTTTCCTTTTTTGAGAAGTC6910.10127005249628482No Hit
TCCCTTAACAGCTGTTCCTATGACGGCTGGGTCACATTCTAATGAGTAAT6880.10083038511931107No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCAGTG17250.0104.770811
AGTGCGT17450.0102.714634
CAGTGCG17600.0101.846693
CGACAGT301.6545586E-596.005131
CGAGTCT607.2759576E-1295.976984
AGTCTGA2050.094.8343351
CTGACAA700.092.549239
TTCGAGT651.6370905E-1188.607132
TTGTCAG502.3783286E-886.4046251
TGAAGCG2350.082.7035755
CTGAAGC2350.082.7035754
TCGAGTC703.274181E-1182.272023
AAGCGAG2400.080.9805767
GAAGCGA2450.079.327926
AGCGTCT1050.075.410498
GTTCGAG903.6379788E-1272.003851
GCGTCTT1150.068.853069
TTACTGG1200.066.0035251
GCGGTTC3850.065.438868
CGGTTCT3850.065.438869
Produced by FastQC (version 0.11.2)