FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_mosquito_1_-_101817_pcr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_mosquito_1_-_101817_pcr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720051
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTAATGGGAGTGAAGGAAAATTTCATAGGCTTGTTGCAAATAGAAAGAA99261.3785134664072405No Hit
CACAACACACCTAGGATGGTTTTGCACAAAATTGATTATCGCAATTATAC30740.426914204688279No Hit
CATCTAATGGGAGTGAAGGAAAATTTCATAGGCTTGTTGCAAATAGAAAG30210.4195536149522742No Hit
CACCTAGGATGGTTTTGCACAAAATTGATTATCGCAATTATACCTTTTCC29070.4037214030672827No Hit
CACACACCTAGGATGGTTTTGCACAAAATTGATTATCGCAATTATACCTT25060.3480309033665671No Hit
GGTGTGGACAGTGATGAGTGAGGAATAAAGAATAACAAATGAGACGTGAG22310.3098391641703157No Hit
TAATGGGAGTGAAGGAAAATTTCATAGGCTTGTTGCAAATAGAAAGAAAA18000.24998229292091811No Hit
GTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCA16060.22303975690610803No Hit
TCCCCACACACACCTAGGATGGTTTTGCACAAAATTGATTATCGCAATTA15950.221512087338258No Hit
GTGATGAGTGAGGAATAAAGAATAACAAATGAGACGTGAGGAATGAGGAA15880.2205399339768989No Hit
GGCTTGTTGCAAATAGAAAGAAAACGGCAGCTGCCAAGAATTCGCAACAC14240.19776376951077074No Hit
GTGTGTACAACTAGTGCAAATGCCGAAAGTACCGATATATCATCAGTGCG13470.1870700825358204No Hit
GTGTTTACAACTAGTGCAAATGCCGAAAGTACCGATATATCATCAGTGCG13160.18276483193551568No Hit
AATGGGAGTGAAGGAAAATTTCATAGGCTTGTTGCAAATAGAAAGAAAAC12310.17096011254758345No Hit
ACACACACCTAGGATGGTTTTGCACAAAATTGATTATCGCAATTATACCT11980.16637710384403326No Hit
GTTTACAACTAGTGCAAATGCCGAAAGTACCGATATATCATCAGTGCGTA11710.1626273694502195No Hit
TATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTG11620.1613774579856149No Hit
TTGTACACACTTGCTTTCCACTTTACTGCTCTGCTTGCTTTTTTGTGTTG10610.14735067377171895No Hit
GGTGTGGACAGTGATGAGTGAGGAATAAAGAATAACAAATGAGGCGTGAG10550.14651739946198256No Hit
TTTCATAGGCTTGTTGCAAATAGAAAGAAAACGGCAGCTGCCAAGAATTC9030.12540778361532723No Hit
CCTAGGATGGTTTTGCACAAAATTGATTATCGCAATTATACCTTTTCCCC8850.12290796068611808No Hit
GTTGCAAATAGAAAGAAAACGGCAGCTGCCAAGAATTCGCAACACAAAAA8700.12082477491177708No Hit
TGGTTGAGGTTGCCGGAGGAAGCAGACGAGAAATATTTCAAGTGCAGTGA8580.1191582262923043No Hit
GTATAAAGGTGCTGTGTCCATACACCAGCACTATGATGGAAACCATGGAG8260.11471409664037686No Hit
CACCACACCTAGGATGGTTTTGCACAAAATTGATTATCGCAATTATACCT8090.11235315276279041No Hit
GGACAGTGATGAGTGAGGAATAAAGAATAACAAATGAGACGTGAGGAATG7960.1105477250916949No Hit
TCCCCACACTTACTCACTCTTCGCTTTTATTATTGAATCGTGGATTGCTC7950.11040884604007217No Hit
ATCTAATGGGAGTGAAGGAAAATTTCATAGGCTTGTTGCAAATAGAAAGA7920.10999220888520396No Hit
CCTTTATACAGAAGGCCCCTGGTCTTTTTTCAAGCCAGTCCCCCACCATA7780.10804790216248572No Hit
ATTATACCTTTTCCCCAAAACAGATTACGCACTGATGATATATCGGTACT7740.1074923859559948No Hit
ACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCCAG7610.10568695828489925No Hit
CACTTACTCACTCTTCGCTTTTATTATTGAATCGTGGATTGCTCAACGAT7520.10443704682029467No Hit
TTGTAAACACTTGCTTTCCACTTTACTGCTCTGCTTGCTTTTTTGTGTTG7420.10304825630406735No Hit
AAATTGATTATCGCAATTATACCTTTTCCCCAAAACAGATTACGCACTGA7330.10179834483946275No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCCAC14100.090.8764341
CATCTAA6700.083.8050461
CCCCACA15700.081.615142
TGGGAGT19150.079.311086
TAATGGG22450.075.353233
CCCACAC18100.070.793243
TGTGGAC18800.066.6256943
CTAATGG25300.066.58032
GGGTGTG6250.064.49981
AATGGGA26450.064.225364
GGGAGTG24500.062.2859347
GGACCCT1650.061.0751236
AACACAC8200.056.1805954
CACGCCC902.1678716E-855.9855237
TCTAATG30600.055.283661
CAGTGAT11550.054.8391539
GAGTGAA28400.054.489179
ATGGGAG28550.054.206655
CACAACA8800.051.535711
GACCCTT2000.050.3869747
Produced by FastQC (version 0.11.2)