FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_mosquito_19_f4_pcr2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_mosquito_19_f4_pcr2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341187
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGAGATGGCTACTGCTATGAGTAACACTTGTCCCATCTTCTTCTC13140.3851260452479139No Hit
GGTCTGAACAGACCCTAGCATCCATCCATCTCGGTTTGAGCACTCTCTTC4910.14390935176310937No Hit
GGAGCATGATCCCTCCACAAGTAGCCAGGCCCGCTCTCCATGCCACGAGC4860.14244387974922842No Hit
CAGTATGACACCAAGTCCTGCTTGACATCCCCCCAGTATGGATCAAGTCT4770.1398060301242427No Hit
CATTAATAGGGTCTACTACCCTCACAGCTGTCAATCCCAGGGCCATGACA4450.13042700923540462No Hit
CTATTAATGTGGTAGGACTACTGTTACTCACAAGGAGTGGGAAGCGGAGC4420.12954772602707607No Hit
AGATAAGGCCCAGGAAAGAACCAGAGAGCAACTTAGTGAGGTCAATGGTG3990.11694466670769989No Hit
TACTACAACCCATCAGAGTCCCTAATTACAATCTCTACATCATGGATGAA3870.1134275338743856No Hit
GGATGAGAGTGGTGATTTCTCCTTGGTAGAGGAAGATGGTCCACCCATGA3800.11137587305495227No Hit
CCCCGTGAGAGCATGCTGCTAGCCCTGGCTTCGTGTCTTCTGCAAACTGC3550.10404851298554751No Hit
TACACATACCACGCTCCTGCAGCAAAAGGTATAGCTATTGGGTTCATGCC3530.10346232417999512No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTGC9050.082.732082
CTGTGCC10400.071.982263
ACTGGAG8350.060.3444143
GCCTGAC607.0910155E-659.9852187
TGTGCCT12500.059.8892444
CTGGAGA8550.058.9328464
TGGAGAT9200.056.3339465
CGGATTG400.006221385753.9866949
CTCGGTC953.3132892E-853.039568
TGCCTGA5100.047.9952132
ATCTAGA605.114486E-447.995211
AGATAAG1900.045.4691431
TGCCTGG8350.044.8338552
AATGTTG1151.4905527E-743.8152925
GCCTGAA1653.092282E-1143.6256143
CAGAGCG1002.8667291E-643.1893549
CCTGCCG855.564428E-542.3425064
ATACCCT855.564428E-542.3425068
GAGATAA1054.0053674E-641.1387521
GAGCTAA700.001093544641.132726
Produced by FastQC (version 0.11.2)