FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_mosquito_19_e8_pcr2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_mosquito_19_e8_pcr2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences538952
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGAGATGGTGAGAGGCAGGAGTTACTCTAATGAACATATGTGTCC95131.7650922531134496No Hit
GCACTGGAGATGGAGCGTGCACCACTTGGCTCTGCTCTGCTTCTTATGAA66001.2245988511036234No Hit
GCACTGGAGATGGCGGTCAGGGACAGCCAGCCTTTTACCCTAGATGGTTC38460.7136071486885659No Hit
GCACTGGAGATGGCTGGCACAGGCCATCAGACTCCCTAGAAAGCAGCAGT33600.6234321423800264No Hit
GCACTGGAGATGGGAAACAAGGACAGATATAAATACAGAAAGCATCTATT27210.5048687081595392No Hit
GCACTGGAGATGGCTACTGCTATGAGTAACACTTGTCCCATCTTCTTCTC26250.48705636123439566No Hit
GCACTGGAGATGGAGATGCCGAGGACGAATGAGTTGCAGATGCAGTTGGG23350.4332482298980243No Hit
GCACTGGAGATGGACCTGGCCCGCCTTTTGCTGCCACACAGCTTGATGGT23290.4321349582152028No Hit
GGAGATGGAGCGTGCACCACTTGGCTCTGCTCTGCTTCTTATGAAGACAT17430.3234054238596387No Hit
GCACTGGAGATGGGAAGCAAGACCCGGCTTCGTGTGGAAAGACCAGCTTC12280.22784960441746202No Hit
GCACTGGAGATGGCTCAGCGGTAAAGACTCTTGCCAGGAAAAGCCTGAGT10820.20075999346880613No Hit
GTCTTGAGGCATCGAGGATGCTGATTGTCTGTGTCTGTGCACAGCATCTG10180.18888509551871038No Hit
TTTCTAACCTACACCTCATGAGGCTCTGGTGAGAGAAGGAATAAGGAGCA9390.17422701836156096No Hit
GAGATGGAGCGTGCACCACTTGGCTCTGCTCTGCTTCTTATGAAGACATG9070.1682895693865131No Hit
GCACTGGAGATGGTGGAGGCAGAGGGATCAGGCATTCATAACTTCAGCTA9060.16810402410604283No Hit
GGTTAGAAATGACCCAACAGCCAGCAGGTGGCAAAGGCTACCATCAGCCT8470.15715685255829834No Hit
GAACATATGTGTCCACTAAGCAAAGATGGCCGGGATACAGTGTCCGATGG8270.1534459469488934No Hit
GGAGATGGAGATGCCGAGGACGAATGAGTTGCAGATGCAGTTGGGCCTGA7620.14138550371832742No Hit
TCCTTAGAGGGTACTCCCTGGAAAAGTACAGTGTATCCTAAAAATATAAA7370.13674687170657127No Hit
GTCTACGACAATCCTTAGAGGGTACTCCCTGGAAAAGTACAGTGTATCCT7270.13489141890186881No Hit
CCTCAAGACTGCTCCTTGGTGAATTATAAGCATATAAAGGAAAGCTTTGC7160.1328504208166961No Hit
GTTTAGGAGAGGATGGAAATTTGTTTCACACCTGGCTGCTCCTTATTCCT7040.13062387745105317No Hit
GGAGATGGTGAGAGGCAGGAGTTACTCTAATGAACATATGTGTCCACTAA7010.13006724160964242No Hit
GCACTGGAGATGGCTGAGCCGTCTTTCTAGCCATCTTTCTACTGTAAGAT6770.1256141548783565No Hit
CCTTATTCCTTCTCTCACCAGAGCCTCATGAGGTGTAGGTTAGAAATGAC6650.12338761151271356No Hit
GCACTGGAGATGGCGGAAGGGAGAGAAGATGGGTCCCTGGATTTTACTGA6610.12264543039083259No Hit
CTCCTAAACCAAGCTCTTTCCTGGGAAAGATGGCCAAGGCGAGACCTAGA6610.12264543039083259No Hit
GGTCTGAACAGACCCTAGCATCCATCCATCTCGGTTTGAGCACTCTCTTC6260.11615134557437397No Hit
GCACTGGAGATGGTGAGAGATGTACTCAGAACATCCCAGTAAACTCTTTC5870.10891507963603439No Hit
GCTTATAATTCACCAAGGAGCAGTCTTGAGGCATCGAGGATGCTGATTGT5820.10798735323368314No Hit
ACTGGAGATGGAGCGTGCACCACTTGGCTCTGCTCTGCTTCTTATGAAGA5780.10724517211180218No Hit
TCCCATGACAGCCCGCATGGGGACAGAGTAGGTTTGCTGGCTGCTTAGGG5710.10594635514851045No Hit
GCACTGGAGATGGTTCAGTGGGTAGAGGCTCTCATCACCAAACCTGACAG5530.10260654010004601No Hit
GATATAAATACAGAAAGCATCTATTCTGTTTCATGAAAGTGAGATGAATG5430.10075108729534356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATGGCG6900.0116.8154149
ACTGGAG64250.0116.646063
GCACTGG66700.0111.971281
CACTGGA70700.0106.227752
TGGAGAT72050.0103.918255
CTGGAGA75150.0100.589534
GGAGATG80600.093.787886
GAGATGG83800.088.7989357
AGATAAG4700.085.811191
GATGGGA9000.076.764419
CTACGAG406.8958485E-571.993363
AGATGGG17000.065.1933068
GCTACGA451.2343616E-464.005982
CACTCGA350.003663353661.7200552
AGATGGT32100.060.5326848
AGATGGA37400.058.8817988
GGCTACG502.0764582E-457.616091
GATGGCT22800.056.5001269
TACGAGA553.3310102E-452.358814
GATGGAC8150.051.2155239
Produced by FastQC (version 0.11.2)