FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_mosquito_18_pcr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_mosquito_18_pcr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences828578
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTAACATAGGCAACATGAGCTCTAAGCTGAGCAGATGCAGTATCGCG53480.6454431568301355No Hit
GTCAGTGCGTGGCTCCAGGTGCGGATGTCTGAAGGACCACTCAGGAACCT27990.3378076656633413No Hit
GTGCGTGGCTCCAGGTGCGGATGTCTGAAGGACCACTCAGGAACCTTAGT27220.3285146359184048No Hit
GTCTTAGGTATGGGTGTTGTCTTACTGCGTTCTTTGGAGGCATGATTTTG25010.30184243366345714No Hit
GTGCGTGGCAAACTAGATGTTGGTAACACGAACAATAGCGACATTAGCAT22130.2670840886434349No Hit
AACTAGATGTTGGTAACACGAACAATAGCGACATTAGCATTCTTAGGTTA22030.26587720166357304No Hit
TTCTTAGGTTAATGATTCTACTGTCTTAACATAGGCAACATGAGCTCTAA17880.21579139199930483No Hit
GGACTAGATCGCGCATGAGCTTGGTGTGTTTATTTTGAATTTCATTGGCA17350.20939489100603687No Hit
GGCTCCAGGTGCGGATGTCTGAAGGACCACTCAGGAACCTTAGTGCAAGC16300.19672257771748708No Hit
CTTAAGAACTGTGGATATTTTGCAGATCTAATTAAGAAAAATGTTTTGAC13960.168481422388719No Hit
ATCTAAGCTTGTCCATTTTTAGGCGGCATTTCAAGTGGCCAGAGAACAGC13960.168481422388719No Hit
GTTCTTAAGCGTGTGATTCAAAATACACAAGTTAAAGATTATTTTTCAAT13930.1681193562947604No Hit
GTGCGTGGCTTGGGGTGGGGCTTACCGCTCGGAGCTGCCGGTCCGAGGTG13890.16763660150281567No Hit
CCTTAAACCTGTCTTGTAACCTTGATACTTACTTTCAGCCTCCATGTGTC13840.16703315801288474No Hit
GTCAGTGCGTGGCAAACTAGATGTTGGTAACACGAACAATAGCGACATTA13640.16461938405316096No Hit
GGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCT12560.1515850046706526No Hit
ATCATAATCTCTGCGATTAACCATCACCTTGTTAAACGCTCATACCTTCA12460.1503781176907907No Hit
GTATTACCTGACCATGAACAATAAGCATTGGTTGGTGCACAAAGAGTGGT12230.1476022776371084No Hit
GTCATAGGAGTTGGGGACAAGAAAATCACCCACCACTGGCATAGGAGTGG12150.14663676805321887No Hit
CTCAGGAACCTTAGTGCAAGCTTATCTTGGAACACTTTTAGCAATTCCAT12010.14494712628141226No Hit
GGTAATACAGATCTGAAAAGTCAAGGCCTGTCCTTGGTTCACAGTCAAGT11990.14470574888543986No Hit
GGCTTGGGGTGGGGCTTACCGCTCGGAGCTGCCGGTCCGAGGTGCCCAGA11890.14349886190557798No Hit
GTCTGAAGGACCACTCAGGAACCTTAGTGCAAGCTTATCTTGGAACACTT11520.139033380080089No Hit
GCGTGGCTCCAGGTGCGGATGTCTGAAGGACCACTCAGGAACCTTAGTGC11420.13782649310022713No Hit
GTCAGTGCGTGGCGCAATTTGACTGCCGTCTTGCGCATGCCCTTCTACAC11110.1340851434626553No Hit
CCTTAGTGCAAGCTTATCTTGGAACACTTTTAGCAATTCCATGTCTTGCT11050.13336101127473815No Hit
ATGCTGGATGAGGGAGTGGAACCAGATGATGTCGATTGCTGGTGCAACAC10880.13130930340897298No Hit
AGCTTATCTTGGAACACTTTTAGCAATTCCATGTCTTGCTTCTCGTACCG10490.1266024441875116No Hit
CTGAAGGACCACTCAGGAACCTTAGTGCAAGCTTATCTTGGAACACTTTT10460.12624037809355304No Hit
AGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTAATTTGGATTTG10450.12611968939556686No Hit
ATCTAGTCCATGAGGTCGTCGTACATGCCCGGTACGAGAAGCAAGACATG10340.1247921137177188No Hit
GCTTGGGGTGGGGCTTACCGCTCGGAGCTGCCGGTCCGAGGTGCCCAGAG10270.12394729283181546No Hit
GTCTTTAGCTGGTCCACTCAGCCACCACAACACCAGAGAGGGTTACAGAA10230.12346453803987073No Hit
TCTTAGAACACGTAAACTTGGCACATGTCACCAAGCTCCCTTTGCCAAAA10220.12334384934188454No Hit
TGTCTGAAGGACCACTCAGGAACCTTAGTGCAAGCTTATCTTGGAACACT10170.12274040585195359No Hit
CTTTTAGCAATTCCATGTCTTGCTTCTCGTACCGGGCATGTACGACGACC10170.12274040585195359No Hit
GTATATGACTTTTTGGCTCGTCGAGCTTCCCAAAAGCCAGGCAATGGCAA10110.12201627366403645No Hit
TTCATATCCTGTATCATTGACAATCATCCCGCTATGCTGGGAGCCATGCA10030.12105076408014696No Hit
TAATAAAGAAGTTCAAGAAAGATCTTGCTGCCATGTTGAGAATAATCAAT9790.11815423532847844No Hit
TCCTTGTAGAGTGAGAAGGGAGCGTCACGGCTCTTCTAGATCGCCGTGCC9650.1164645935566718No Hit
GGCATGTACGACGACCTCATGGACTAGATCGCGCATGAGCTTGGTGTGTT9490.11453357438889278No Hit
GTAGTATGCACTCCCGCGTCTAGTGATCTCTGCTGCCATGGCTGTAGTCA9400.11344737610701708No Hit
GATTATGATTGAAAAATAATCTTTAACTTGTGTATTTTGAATCACACGCT9230.11139566824125188No Hit
TCCGTACACAACCCAAGTTGATGTCGTGTTGCACCAGCAATCGACATCAT9220.11127497954326569No Hit
CTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTA9090.10970602646944524No Hit
TTAGTGGACAGAGGTTGGGGAAACGGTTGTGGACTTTTTGGCAAAGGGAG9070.10946464907347286No Hit
ATTCTACTGTCTTAACATAGGCAACATGAGCTCTAAGCTGAGCAGATGCA8980.10837845079159716No Hit
CTCCAAGAGAGAAGTGGTCCATATGATCGGTTGACCCCGCTGTCACCATT8970.10825776209361097No Hit
GCTCCAGGTGCGGATGTCTGAAGGACCACTCAGGAACCTTAGTGCAAGCT8950.1080163846976386No Hit
TGTCAGTGCGTGGCAAACTAGATGTTGGTAACACGAACAATAGCGACATT8860.1069301864157629No Hit
ACTAGATGTTGGTAACACGAACAATAGCGACATTAGCATTCTTAGGTTAA8680.10475778985201151No Hit
CAATAGCGACATTAGCATTCTTAGGTTAATGATTCTACTGTCTTAACATA8640.10427503506006677No Hit
ATTCCATGTCTTGCTTCTCGTACCGGGCATGTACGACGACCTCATGGACT8530.1029474593822187No Hit
GTGCGTGGCACGGCCATTGCTCGAATTGCCAACCAGGCCAAAGCAAATCC8490.10246470459027393No Hit
TGATTCTACTGTCTTAACATAGGCAACATGAGCTCTAAGCTGAGCAGATG8370.10101644021443967No Hit
TCCTAGAGGAGAATGGAGTTCAACTGACAGTTGTTGTGGGATCTGTAAAA8340.10065437412048112No Hit
ATCTAGTTGTAGCATAAAGGGGACGATATGTTGTTGCGTAGGGTAGACTC8340.10065437412048112No Hit
CTTCTAGATCGCCGTGCCTCACCTTTTTTGTGATGACAGGTTCCGTACAC8300.10017161932853635No Hit
CTCAATATGTCTGCAAAAGAACATTAGTGGACAGAGGTTGGGGAAACGGT8300.10017161932853635No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCAG151.2636313E-4144.019321
CGTCAGA151.2642391E-4144.001942
GTCAGTG12350.0100.288761
CAGTGCG12250.099.307973
AGTGCGT12400.098.106664
TTCGAAA607.2759576E-1296.00132
GTTCGAG1400.092.583851
GTTCGAA554.3837645E-1091.648661
TCGAGTC1450.089.358943
GATGTGT259.6565264E-486.38037
TTGTCAG608.749339E-1084.011271
GGCAGTT1050.075.411387
CAGTTCG406.902679E-571.983594
AGTCTGA2550.070.597711
GTGGCAG1450.069.5013965
TGCGTGG22500.064.640872
GTGCGTG22650.063.9026151
GCAGTTA1250.063.345568
CGTGGCT11400.062.5120624
CAGTTAG1300.060.9091879
Produced by FastQC (version 0.11.2)