FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_lp_negative_control_cb10-14_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_lp_negative_control_cb10-14_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23.4482758620689653No Hit
AGTTCGCTTTCAGTATGATGTTCTTATCCATGATCGCCTGGTCCATTCTG23.4482758620689653No Hit
ATTGTCATTGTGTCAATGTCAGTTACCACTATTCCATCCACAACGGGATT11.7241379310344827No Hit
CTCTTGGAGTGCTTGTGATTCTACTCATGGTGCAGGAGGGGTTGAAGAAG11.7241379310344827No Hit
CAAGGTCCCATGCATAAAATGGCCTCCCTTTGCCAATGCCAAACAGCACT11.7241379310344827No Hit
GAAGATGGTGCTTTGATGGCACAACCAACAACACCATAATGGAAGACAGC11.7241379310344827No Hit
ATTACAAAACCTTACTATGAAAGTATCTAGACTTATTCATGTGGCTAACT11.7241379310344827No Hit
GTCCAACATTCGGTAACCAATTCATAAAACAACTACACCTTAATGCCGAT11.7241379310344827No Hit
GTGATCAATAATGGAAGCTATGTTAGTGCTATAAGCCGGGGAAAGTGGGA11.7241379310344827No Hit
TCTCACAGTCGGAGTCGCGCTAGTTGTCATAGCGGATCCATGGGTTATAG11.7241379310344827No Hit
GTGATGCCCCATTCCATGATAGGCTAATCCCACAACAAAACACCAAAAAA11.7241379310344827No Hit
AGAGATCTCATACTCACGGTCTTACTGATGGCCGTGTGAGTGATGAACCC11.7241379310344827No Hit
CTGATTACCAACCAAATGGAGGAAGGGCACAGAACTCTGGCGTTGGCCGT11.7241379310344827No Hit
CTCCTAGAGTCTAGGCCCCGGTCATCCTGGAAGTTGGCTCCCATCTCTGA11.7241379310344827No Hit
CCTGGCATGACCGATGTCAAACGAAATGGGGTGGGGTGTGACATGTGAGG11.7241379310344827No Hit
TGATTGGTGAGTTAGAGTCAGGAAACGCATCACGGGTTCCTGGTGGTGTG11.7241379310344827No Hit
ATGACAAAGTCCCACTCTTGATTTTTTGTTTTCTGAAATTCTGTCTCAAA11.7241379310344827No Hit
GTAATAATCAGTAAGAGGGAAAATGAAATCATAAAAGTTCAAATCAACTA11.7241379310344827No Hit
CAATAGTGACAGCAGCAACAACCCAACATATGGAATAATTCTCACATTTA11.7241379310344827No Hit
CTATCATCCCGTCAGGCCCCCTCAAAGCCGAGATCGCGCAGAGACTGGAA11.7241379310344827No Hit
GAGCAACTAAGTGAGCTCAATGGTGACAGCGGGGTCAACAGATCATATGG11.7241379310344827No Hit
TCACTGGAGATGGCTACTGATATGAGTAACACTTGTCCCATCTTCTTCTC11.7241379310344827No Hit
GCACTGGAGATGGCTACTGATATGAGTAACACTTGTCACATCTTCTTCAC11.7241379310344827No Hit
ACCTCAAGTTGGACAGCTGGGGTGATGAGAGATGTCAATGCGGCATAGAT11.7241379310344827No Hit
CTCCATCTGTGGTCTCTCCTTTCTTCACTTCTTTGGGAGCAGGCACGTCC11.7241379310344827No Hit
CAGATGAGGACCCCCCCTGCATTTTTGAAATCCAAATAACTATTTAATGG11.7241379310344827No Hit
GGTTTGAGATATTCCCCAAGACAAGTTCATGGCCCAATCATGACTCGAAC11.7241379310344827No Hit
GAGCACCACCAGTAATAAAAACACAACAATGAGGACACATGCAATTCTGG11.7241379310344827No Hit
GGCTAGTCCTGTAACTCCAGTCACCACGTCCCAAGGCTTTGACAGGAGTC11.7241379310344827No Hit
AGGACACATGACAGAGAGGTTTCAGGAAGCCATTGACAACCTCGCCGTGC11.7241379310344827No Hit
CACTACTACTGAAATCTAATTAACAGAACTCTCATCTGACAATAGAATTT11.7241379310344827No Hit
GAAGAGAAGTTCGACTTTTGAATAATTCCAATTGCTCCCAGTTGCAAGGT11.7241379310344827No Hit
GGAAGGAAGCGATCCAGCGGCTACAACCACACCCAAGAAGAACAACTAGG11.7241379310344827No Hit
ATCAGAGTCAGGGGTGTTATTTGTGAATAGCAAACCAACATTAGCAGCGG11.7241379310344827No Hit
CTATCAGGGAGAGGAGAATGATCCCTCCACAAGTAGCCAGGCCCGCTCTC11.7241379310344827No Hit
GTGTTGAACCTAGCAGTCTGCGAGTCATCACTCTGTACACTCCATCTGTG11.7241379310344827No Hit
ATTCTGTCTCAAAAGTCTTACTGGTGCGCTGTATGCCACGCTATCCAGCC11.7241379310344827No Hit
GAGTGAAGGAGACCAGCAAGGCTGGTCTGACTTTAAATGCCGATAACAAT11.7241379310344827No Hit
GTACATGTAGTGCGCCACAAGCAGAATGATAGCTACTATCAGAGTCAGGG11.7241379310344827No Hit
CCACTGGATAAGGCTACAGCTATAAGTAACACCAGTCGAATATAAAGAAC11.7241379310344827No Hit
GGTGTGGGGAAACGCAAGCGGCCACGCGTCTGCACCAAAGAAGAGTTTAT11.7241379310344827No Hit
TTAACAGCCGAGCACAGCGCATGTGCCGGCGGGCGAAGTGAGCTTCTGTG11.7241379310344827No Hit
CCACAAGCAGAATGATAGCTACTATCAGAGTCAGGGGTGTTAATTGTGAA11.7241379310344827No Hit
GTAATTGTGTCAATGTCAGATACCACTATTCCATCAACAACGGGATTCGT11.7241379310344827No Hit
TCTAAGTTACTTGCGTTTCAAAGAATTCTTCGGGAGACAAAAGTACGTTC11.7241379310344827No Hit
TAACCAAAGAATTAAAAAATCTATACACTGAAAACTAGAAAGCACTGATG11.7241379310344827No Hit
TACTACCGGTCACTCCTGGAGCCGGATTGCCGGGGTTATACCGCTTACAT11.7241379310344827No Hit
ATCAAGGACGGACATCCGACATGCGAACAGAAGTTATAAGAATGATGGAA11.7241379310344827No Hit
CACTTGGCTTAAGGAGATCAAAGTACACCGCACACTGCACACACAACTTG11.7241379310344827No Hit
AGCCATGGCCGTGCCACGCACTGACCACATCGCTCTACCAATGGTGGCTG11.7241379310344827No Hit
CTGCCACACCACCAGGACCCCGAGTGGCGTTTCCTGACACTCCCACACCA11.7241379310344827No Hit
GAACATAACTAGGCTTACTGATCTTCTTGACTCTCCTTGGTATTAACACT11.7241379310344827No Hit
AATATAACCAAGAGAACGCAGAAAAAAATAATTTTGAGAACACACAACTA11.7241379310344827No Hit
ATTCTTTCGGAGCAGGCACGTACCAGAGGGCGCCACGCCTTTTCCCAGTC11.7241379310344827No Hit
GAGGATATTGCACATTCTTTCATAAGCAACCCTTGTCCTTCGTCAATTTT11.7241379310344827No Hit
CCATAAAATGGCATCCCTTTGACAATGCCAAACAGAACTCCAGCTTGAGT11.7241379310344827No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTCTCA50.0144.05
TTCTGTC50.0144.02
TCTGTCT50.0144.03
TCAAAAG50.0144.09
GTCTCAA50.0144.06
CTGTCTC50.0144.04
ATTCTGT50.0144.01
TCTCAAA50.0144.07
CTCAAAA50.0144.08
Produced by FastQC (version 0.11.2)