FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_liver_ifn--_red_needle_pcr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_liver_ifn--_red_needle_pcr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences569813
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTG21810.3827571501527694No Hit
GTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAGGATGTTGCA16290.2858832634566077No Hit
ACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCCAG12390.21743975655171083No Hit
GTATAAAGGTGCTGTGCCCATACACCAGCACTATGATGGAAACCATGGAG11690.20515502454313958No Hit
AATGGGAAGAGTGACTGACTGTTCACAGACCTCAGTGATGTGAGCACTGT9800.17198624811999727No Hit
GTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCA9630.16900281320362995No Hit
GGTGTGGACCCTTCCTCACCCAAGTAGCGGACTTGGGTGGATAGATAGTC9110.15987701228297704No Hit
ACCATGGGATTCTCAATGGACATTGATCTGCGGCCAGCCTCCGCCTGGGC8650.15180418839163023No Hit
ATATAAAATAGCTATTATAAATGCACATAGTGTATTCTATAGCTGCCAGG8650.15180418839163023No Hit
ATCTATGCCGCATTGACAACTCTCATCACCCCAGCTGTCCAACATGCGGT8640.15162869222007921No Hit
GTACCAGATGGCGCGGCTACCTTTTGCTTTCCCGAACTCTCCTTGCTTCT8630.1514531960485282No Hit
TACCAAAACAAAGTGGTGAAGGTTCTCAGACCAGCTGAAGGAGGAAAAAC8280.14531083004424258No Hit
CTGTTACACTGTGGCTAAAACTTGTATCTTCAACCTTTGAAAAAGCCCAC8240.14460884535803853No Hit
TTCTATCACAGTGATGTATGGTTAAACACTTGGATCAAGTCATAACCAGT8230.14443334918648748No Hit
ATACAGGACATGGGCCTACCATGGGAGCTACGAAGCCCCCACGCAAGGAT8120.14250289129942628No Hit
GAGTAACACTTGTCCCATCTTCTTCTCCACCTGGGGGTCTATTGTCATTG8070.1416254104416712No Hit
CCTATGATCCTGCGCACCATGTTGACTGTGTCTTTGATGTTTTCAGCCCA8050.14127441809856917No Hit
ATCTAATGGGAAGGAGAGAAGAAGGAGCAACCATGGGATTCTCAATGGAC7900.1386419755253039No Hit
ATCTGGTACATGTGGTTGGGAGCCAGATTCTTGGAGTTTGAAGCTCTTGG7610.1335525865503244No Hit
GTCCTGTATGGGTGGTTTTCATCAAGAAACCATGTTTCTGCATGTTCATT7600.13337709037877338No Hit
ATTCCACACCATGAGCATGTCCTCAGTGGTCATCCATTCTCCCTTTCCAT7490.13144663249171218No Hit
TTCTAGAAGAAATGAATCGGGCACCAGGAGGAAAGATGTACGCAGATGAC7240.12705922820293675No Hit
GGTTAAGAGACGTGGAGGTGGGACGGGAGAGACTCTGGGAGAGAAGTGGA7070.12407579328656945No Hit
TCCCTTATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGG7070.12407579328656945No Hit
ATTAAATACACATACCAAAACAAAGTGGTGAAGGTTCTCAGACCAGCTGA7070.12407579328656945No Hit
GTATTTAATCACGGCCAACGCCAGAGTTCTGTGCCCTTCCTCCATTTGGT6990.12267182391416132No Hit
TCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTGGG6950.12196983922795725No Hit
GTGTACAACATGATGGGAAAAAGAGAAAAGAAGCAAGGAGAGTTCGGGAA6840.12003938134089605No Hit
CCTTAATGGCGATGGCCACACAAGCTGGAGTGCTGTTTGGCATGGGCAAA6810.119512892826243No Hit
CCATAAGTCCTCCCTTTTTCCTAGATAGGGAATGTCTGTCCATTTTGTTA6420.11266854213575332No Hit
GTGACTGACTGTTCACAGACCTCAGTGATGTGAGCACTGTGGCTCAGGAG6170.10828113784697786No Hit
TTCCTAGATAGGGAATGTCTGTCCATTTTGTTACAGGAGTCTTGTCCTCC6090.10687716847456973No Hit
AGTACATGTAGTGCGCCACAAGCAGAATGATAGCTACTATCAGAGTCAGG6040.10599968761681464No Hit
ATCCACTAGGGCCCAAAACCTTGGATCATTCACAGCTTCCACAGCCGTCT5990.10512220675905956No Hit
GACTAATCCTCCCCCATGCCTACGTTGCAGTCGCTCCATGGTTTCCATCA5830.10231426801424327No Hit
CCTTTATACAGAAGGCCCCTGGTCTTTTTTCAAGCCAGTCCCCCATCATA5820.10213877184269224No Hit
CTACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCC5820.10213877184269224No Hit
CTCCAGTGCTGTGCTGCTGCGGACCGCCTGGGGATGGGGGGAGGCTGGAG5790.10161228332803919No Hit
GTAATGAACATAGTCTCTTCCTGGCTGTGGAAGGAGCTGGGGAAACGCAA5770.10126129098493716No Hit
TCCCAGGAGGAGCTGACTTGTGGTGGACACACTTTTTATGATGTTGCTCT5770.10126129098493716No Hit
GGTGTGGACAAAGAGGAAGAGGATGAAGAAGGAGAGGATGAAGAGGAAGA5730.1005593062987331No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGGGT900.0103.981482
TCCCCAC5700.095.991341
CCGGGTG1100.085.068293
CCCCACA6850.078.8180852
CCCACAC7250.075.45583
CATCTAA2500.071.99351
ATTCCGG300.001989889771.99351
TGTGGAC9600.065.982463
CGGATTT451.2376942E-463.9717529
CTCCGGG1500.062.3943631
ATCTAAT4600.061.0379641
CAGGCGT1102.7284841E-1158.883099
TGGACCC2950.058.56075
GTGGACC3950.058.3136064
TCTAATG4850.057.8865972
CTCTATC1101.7807906E-952.358911
GAGACCG859.382493E-750.8010989
CCACACG1004.9722075E-850.38664
CTAATGG5600.050.1295243
GACCCTT3500.049.3539737
Produced by FastQC (version 0.11.2)