FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_liver_ifn--_red_needle_pcr2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_liver_ifn--_red_needle_pcr2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences636406
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGAGATGGCTACTGCTATGAGTAACACTTGTCCCATCTTCTTCTC96361.5141277737796313No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT37380.5873608985458968No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA26650.4187578369782812No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC22840.35889039386806537No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG21980.3453770077591978No Hit
GAGTAACACTTGTCCCATCTTCTTCTCCACCTGGGGGTCTATTGTCATTG19270.3027941282766033No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT19050.299337215551079No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA18010.2829954463031461No Hit
CATACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAG17570.2760816208520976No Hit
GCTATGAGTAACACTTGTCCCATCTTCTTCTCCACCTGGGGGTCTATTGT16470.2587970572244762No Hit
GTGCCTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCG15750.24748352466821494No Hit
ATCTATGCCGCATTGACAACTCTCATCACCCCAGCTGTCCAACATGCGGT15690.24654073028852652No Hit
GTTCAGACCATGCGGCCCTGAAGTCGTTCAAAGAATTCGCCGCTGGAAAA15620.2454408035122233No Hit
GTGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGCATCT15430.24245528797654328No Hit
ACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCCAG14820.23287021178304418No Hit
AGATAAGGCCCAGCAGAGGTGTGGACAAAGTATGGAGAGAAGAGAGTGCT14170.22265660600308607No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA14000.2199853552606355No Hit
TCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAA13550.21291439741297222No Hit
AATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACCTTTTGCATAAT13190.20725763113484158No Hit
CAGCAGAGGTGTGGACAAAGTATGGAGAGAAGAGAGTGCTCAAACCGAGA12890.20254365923639941No Hit
CCTTAATGGCGATGGCCACACAAGCTGGAGTGCTGTTTGGCATGGGCAAA12380.19452990700904768No Hit
ATCTGTGCCTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGT12160.1910729942835234No Hit
TTATAAGGCAGCGGCAGCCCAACTGCCGGAGACCCTAGAGACCATTATGC11890.18683041957492544No Hit
AGTACATGTAGTGCGCCACAAGCAGAATGATAGCTACTATCAGAGTCAGG11850.18620188998846648No Hit
CTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAA11700.1838449040392454No Hit
AACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCCGAAACCA11480.18038799131372113No Hit
GGAGATGGCTACTGCTATGAGTAACACTTGTCCCATCTTCTTCTCCACCT11260.17693107858819684No Hit
AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT11050.1736312982592873No Hit
GGTATGAGCACCACCAGTAATAAAAACACAACAATGAGGACACATGCAAT10270.1613749713233376No Hit
ATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATAAT10260.1612178389267229No Hit
GTACATGTAGTGCGCCACAAGCAGAATGATAGCTACTATCAGAGTCAGGG10140.159332250167346No Hit
GCATAAAATGGCATCCCTTTGCCCATGCCAAACAGCACTCCAGCTTGTGT9900.15556107264859226No Hit
GGTCTGAACAGACCCTAGCATCCATCCATCTCGGTTTGAGCACTCTCTTC9890.1554039402519775No Hit
AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG9830.1544611458722891No Hit
GCTCATACCCGAGCCAGAGAAGCAAAGATCTCCCCAAGATAACCAGATGG9670.15194702752645323No Hit
GGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTTTTTTCCGTTC9610.1510042331467648No Hit
GGTGATAGCTGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTG9410.14786158521447001No Hit
ACCTAAAAACAATTTTATGAATCAACTCGTCTATGTGGCAAAATAGTGAG9400.14770445281785527No Hit
GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT9320.14644739364493736No Hit
ATTTTATGTTGAGCTTGAACGCTTTCTTTATTGGTGGCTGCTTTTAGGCC9210.14471893728217522No Hit
ACCATGGGATTCTCAATGGACATTGATCTGCGGCCAGCCTCCGCCTGGGC9080.14267621612618359No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA8900.13984783298711828No Hit
GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC8730.13717658224466772No Hit
TTCCAGAAGAGCTGTCCCTCTTTTGGCTATAATCTAAACTTACTTTTTGA8680.136390920261594No Hit
GAATAAGGGCATCGGGAAGATGGGCTTTGGAATGGTAACCCTTGGGGCCA8590.13497672869206134No Hit
GGTGTGGACAAAGTATGGAGAGAAGAGAGTGCTCAAACCGAGATGGATGG8520.13387680191575818No Hit
ATTTTATGCATGGGACCTTGGAGTCCCGCTGCTAATGATGGGTTGCTATT8350.1312055511733076No Hit
ACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAG8250.1296342272071602No Hit
GGCTACTGCTATGAGTAACACTTGTCCCATCTTCTTCTCCACCTGGGGGT8200.1288485652240865No Hit
ATCTGTGCCTGGAGAATTGGAATTCTTGTTACTCATACTAACAGTGTTGC8200.1288485652240865No Hit
CTATCAGAGTCAGGGGTGTTAATTGTGAATAGCAACCCATCATTAGCAGC8150.1280629032410128No Hit
ATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAATTGAAATTTTA7960.12507738770533278No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG7840.12319179894595589No Hit
GAACTAACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCCGA7690.12083481299673478No Hit
ATCTTGGGGAGATCTTTGCTTCTCTGGCTCGGGTATGAGCACCACCAGTA7670.12052054820350531No Hit
GAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAA7650.12020628341027582No Hit
ATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAA7570.11894922423735792No Hit
GCACTGGAGATGGGACATTGATGGTGCTGGTGAATTACATCTACTTTAAA7450.11706363547798104No Hit
GTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTTTAGGAAGT7430.11674937068475155No Hit
TCATACAACAACTACTCCTTAATGGCGATGGCCACACAAGCTGGAGTGCT7350.11549231151183365No Hit
TTTTTAGGTAGAGGTGAAAAGCCTAACGAGCTTGGTGATAGCTGGTTACC7300.11470664952875995No Hit
CCCTTATTCCGCATCAAGACGAAGAAGATCCCCAGTGAAACTGTTCCCAG7270.11423525233891572No Hit
TCCCATGGTTGCTCCTTCTTCTCTCCTTCCCATTAGATGAGCTATGTCAT7200.11313532556261253No Hit
CCCTTTGCCCATGCCAAACAGCACTCCAGCTTGTGTGGCCATCGCCATTA7130.11203539878630937No Hit
CCACTATTCCATCCACAACGGGATTCTTCATGATGCCAGCTGCTGTCCTT7120.11187826638969463No Hit
CATCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAAT7080.11124973680323566No Hit
GAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGT7000.10999267763031775No Hit
CTACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCC6900.10842135366417036No Hit
CCATTAAGGAGTAGTTGTTGTATGAAGTGGTTACCGCATGTTGGACAGCT6890.10826422126755562No Hit
CATCTAATGGGAAGGAGAGAAGAAGGAGCAACCATGGGATTCTCAATGGA6860.10779282407771139No Hit
CGTCTATGTGGCAAAATAGTGAGAAGATTTTTAGGTAGAGGTGAAAAGCC6800.10685002969802297No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC6800.10685002969802297No Hit
GTATGGAGAGAAGAGAGTGCTCAAACCGAGATGGATGGATGCTAGGGTCT6780.10653576490479348No Hit
GATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAAT6640.10433591135218712No Hit
AAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTTCTTTA6490.10197892540296602No Hit
CCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAGA6480.10182179300635129No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGGAG25950.0113.4446643
TGGAGAT26350.0111.7225345
CACTGGA30950.094.885012
TCTGTGC8250.092.480482
CTGGAGA33500.089.3812944
GGAGATG34650.087.453466
CTGTGCC9200.083.713233
AGATAAG5500.075.915711
GCACTGG42150.070.366511
GATGGGG2100.065.117599
TGTGCCT12900.059.7024544
GAGATGG49400.058.8643727
TGCCTGG12150.054.5016562
GGAGAAT5200.048.4465457
GATAAGG8650.048.2625242
AGATGGC38100.047.414678
GAGAATT5450.046.2205928
GATGGCT33700.045.703339
GTCCTAG657.594562E-444.300951
ATCTGTG18650.040.916021
Produced by FastQC (version 0.11.2)