FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_liver_ifn--_red_needle_f11_pcr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_liver_ifn--_red_needle_f11_pcr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1129683
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACAT235522.084832647742774No Hit
ATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTC232892.061551780455225No Hit
CTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAAC159781.4143790780245433No Hit
GCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATG115751.02462372187596No Hit
CATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGC110650.9794783138278614No Hit
ACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTG109160.9662887730451817No Hit
GTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAG96610.8551956610836846No Hit
TGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTG88580.7841137735099137No Hit
GATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGT87880.7779173449542925No Hit
GTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGC71400.6320357126733783No Hit
CGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTG64990.5752941311854742No Hit
GCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCA61740.5465249986058036No Hit
GTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGG59150.5235982129500045No Hit
TGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCA55340.4898719375258369No Hit
GTGCGTGGGAGACCAGACGGTTTCAATGATTGTTTAATGCAAACGACTCT50330.4455232131491755No Hit
AATATACACATTGCACAGATGCACAGTGGAATATATGGATCGAAATCGGC47490.4203834172949402No Hit
GGAATATATGGATCGAAATCGGCACCTAACTACATGCGGCTAATTGCGGT39060.34576071340367165No Hit
GTGGAATATATGGATCGAAATCGGCACCTAACTACATGCGGCTAATTGCG38800.343459182797298No Hit
ATATACACATTGCACAGATGCACAGTGGAATATATGGATCGAAATCGGCA38310.3391216828083631No Hit
ATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCC37130.3286762746717442No Hit
GCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGAC36000.3186734685748126No Hit
GGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGAT35930.31805382571925045No Hit
CACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACA35270.31221147879537886No Hit
GGTAATAGTAGTAGGCTTGAATTTCGCAAAAATCAGTAGTTCAAAAAAGT34950.30937882574138054No Hit
GTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACG33840.2995530604603238No Hit
TTTATTAACAGTAATTTTGGGTTGCCCTTAGCAACGGGTGTTTTGCAATT33730.2985793359730119No Hit
GCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCA33630.2976941318936374No Hit
CAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCG30770.2723772952235273No Hit
TTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGT28920.2560010197550994No Hit
CGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGA28690.25396505037253814No Hit
ATATTAACTATCACCACTGGCAGAGATCTGGGCAGGGTTGGGAAAAGATC27620.24449336672323121No Hit
GTTCTATCATATTACCCAGATCCAGTGCTGGGAATGGTAATAGTAGTAGG27090.23980178510254646No Hit
ATATTACCCAGATCCAGTGCTGGGAATGGTAATAGTAGTAGGCTTGAATT26960.23865101979935965No Hit
GCCCTTAGCAACGGGTGTTTTGCAATTTTCAAGGCTCTTTTCCTACAGCA26080.23086122390086422No Hit
GTGCGTGGCAAACTAGATGTTGGTAACACGAACAGTAGCGACATTAGCAT22650.20049872397831955No Hit
ATGTTGGGTGCATGAATTTTCTAAATCGTTAGTGTCATTGAAGGCTTTAA22640.2004102035703821No Hit
TGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGA20820.18429948932576662No Hit
TGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACA20220.1789882648495197No Hit
AGCCTATGTTGGGTGCATGAATTTTCTAAATCGTTAGTGTCATTGAAGGC19590.1734114791494605No Hit
AACTAGATGTTGGTAACACGAACAGTAGCGACATTAGCATTCTAAGGTTA19390.17164107099071155No Hit
GTCAGTGCGTGGGAGACCAGACGGTTTCAATGATTGTTTAATGCAAACGA19370.17146403017483666No Hit
ACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAA18620.16482499957952806No Hit
GATCTGGGCAGGGTTGGGAAAAGATCTGAAATTCATTCTACAGTAGCTAA18420.1630545914207791No Hit
TCCTACAGCAGCGATTGGCGTGAGTTTCACTGGCTTCGCTGACTGCGATT18080.16004489755090587No Hit
TGGTAATAGTAGTAGGCTTGAATTTCGCAAAAATCAGTAGTTCAAAAAAG17930.15871709143184418No Hit
AACTACATGCGGCTAATTGCGGTTCTGAAAAACCTTAATTTTTCATAAAA17120.15154693838891087No Hit
GTCTACTTAGGTAGGACCCCATAGGGGAACACGGCCCAATATACACATTG17100.15136989757303598No Hit
CTCCAAGAGCCATTGGTAAGTTCGTTGTTTTTAAGCAAATTAATGAACCA16850.14915688737459978No Hit
TTCTAAGGTTAATGCTTCTACTGTTCTATCATATTACCCAGATCCAGTGC16650.14738647921585082No Hit
TTGCACAGATGCACAGTGGAATATATGGATCGAAATCGGCACCTAACTAC16380.14499642820153974No Hit
ACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTT16330.1445538261618525No Hit
TAGTAGTAGGCTTGAATTTCGCAAAAATCAGTAGTTCAAAAAAGTGAATC15930.14101300984435458No Hit
ACTTAGGTAGGACCCCATAGGGGAACACGGCCCAATATACACATTGCACA15700.13897704046179327No Hit
CTTTTCCTACAGCAGCGATTGGCGTGAGTTTCACTGGCTTCGCTGACTGC15440.13667550985541962No Hit
TCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCA14870.13162984660298507No Hit
CCTAACTACATGCGGCTAATTGCGGTTCTGAAAAACCTTAATTTTTCATA14800.13101020374742295No Hit
GCCTATGTTGGGTGCATGAATTTTCTAAATCGTTAGTGTCATTGAAGGCT14300.12658418335055055No Hit
ATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGATGTC14220.12587602008705098No Hit
TTCTACAGTAGCTAAACAAAGTCAATCGCAGTCAGCGAAGCCAGTGAAAC13740.12162704050605345No Hit
GTTTAATGCAAACGACTCTGCTATGTCTACTTAGGTAGGACCCCATAGGG13550.11994515275524195No Hit
GTAGTAGGCTTGAATTTCGCAAAAATCAGTAGTTCAAAAAAGTGAATCTC13540.11985663234730451No Hit
ACCCAGATCCAGTGCTGGGAATGGTAATAGTAGTAGGCTTGAATTTCGCA13450.11905994867586747No Hit
GATGCACAGTGGAATATATGGATCGAAATCGGCACCTAACTACATGCGGC13390.11852882622824278No Hit
TGCTTCTACTGTTCTATCATATTACCCAGATCCAGTGCTGGGAATGGTAA12980.11489948950280741No Hit
CTATCATATTACCCAGATCCAGTGCTGGGAATGGTAATAGTAGTAGGCTT12790.11321760175199591No Hit
GTCAGTGCGTGGCAAACTAGATGTTGGTAACACGAACAGTAGCGACATTA12450.11020790788212269No Hit
GCCCAGATCTCTGCCAGTGGTGATAGTTAATATTTTGGTTCATTAATTTG12310.10896862217099842No Hit
CCATAGGGGAACACGGCCCAATATACACATTGCACAGATGCACAGTGGAA12110.10719821401224945No Hit
ATGTTGTCAGTGCGTGGCACGCACTGACAACATCGTGCCACGCACTGACA11870.10507372422175071No Hit
GCTAAGGGCAACCCAAAATTACTGTTAATAAATGTTCTATTTCCCGCTGC11830.10471964259000091No Hit
AGCTAAACAAAGTCAATCGCAGTCAGCGAAGCCAGTGAAACTCACGCCAA11570.10241811198362727No Hit
TCCCAACCCTGCCCAGATCTCTGCCAGTGGTGATAGTTAATATTTTGGTT11570.10241811198362727No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAG903.6379788E-1271.998081
TTCGATA406.8983965E-571.994892
CGACAGT1250.063.3583071
TGGGAGA16300.056.0792736
GGGAGAC17050.054.0274167
GTCCACG806.1629726E-753.9985581
GAGTCTG2000.053.981825
GTCTGAA1600.053.9746557
TGTTGCC3250.048.7220545
GGAGACC22050.044.3873148
GAGACCA22900.042.425499
CGTGGGA22400.041.7716454
AGTCTGA2150.040.172526
GCAAACT12100.039.254298
CAAACTA13950.034.0485239
CGAGTCT3200.033.738644
GTGGGAG29600.031.6109775
GCGTGGG30100.031.5655173
GGCAAAC15600.030.9085647
GTTCGAC2101.5410478E-830.8563181
Produced by FastQC (version 0.11.2)