FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_infko_m-red_liver_pcr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_infko_m-red_liver_pcr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences390312
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCA14610.3743159318698887No Hit
TATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTG9310.23852712701633566No Hit
ACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCCAG6670.17088892988173565No Hit
GTATAAAGGTGCTGTGCCCATACACCAGCACTATGATGGAAACCATGGAG5610.14373116891102503No Hit
CCTTTATACAGAAGGCCCCTGGTCTTTTTTCAAGCCAGTCCCCCACCATA5320.13630121543790608No Hit
GATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCACAGTG4560.11682961323249094No Hit
GTCCTGTATGGGTGGTTTTCATCAAGAAACCATGTTTCTGCATGTTCATT4440.11375514972637275No Hit
GGTGTGGACCCTTCCTCACCCAAGTAGCGGACTTGGGTGGATAGATAGTC4330.11093689151243108No Hit
ATTCCACACCATGAGCATGTCCTCAGTGGTCATCCATTCTCCCTTTCCAT4070.1042755539158417No Hit
GAGTAACACTTGTCCCATCTTCTTCTCCACCTGGGGGTCTATTGTCATTG4050.10376314333148866No Hit
ATCTATGCCGCATTGACAACTCTCATCACCCCAGCTGTCCAACATGCGGT4030.10325073274713563No Hit
GGTGTGGACGGGAGACCGGGTCGGGGGCGGGTGCCCAGCGCCGAGCACCA3930.10068867982537047No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTAAT1200.077.9940261
TCCGGGT1900.075.783672
TGTGGAC17350.054.759873
CTAATGG1600.053.982033
ATATACC1206.366463E-1153.9820254
TGGACGC553.3338298E-452.3462075
TCTAATG2100.047.996332
GAAGTCG450.00991481947.9840245
ATACCTT1352.0372681E-1047.984026
TGGACGG3100.046.4361535
GTGGACT5850.045.5233084
TAATGGG1900.045.458554
TGGACTC3500.045.2420775
TAAACCT657.5976935E-444.2929424
CCTATAT1505.7298166E-1043.1966931
GGTGTGG22550.042.7816471
CGGAAGT1202.0910375E-741.9698839
ATTCGTA1557.894414E-1041.8032532
TATACCT1557.912604E-1041.7925385
GTGGACC5400.041.3195724
Produced by FastQC (version 0.11.2)