FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_infko_m-black_blood_pcr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_infko_m-black_blood_pcr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences531353
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCA23360.4396324100927255No Hit
CCATAAGTCCTCCCTTTTTCCTAGATAGGGAATGTCTGTCCATTTTGTTA15260.2871913774835185No Hit
TATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTG12960.2439056521747313No Hit
GTATAAAGGTGCTGTGCCCATACACCAGCACTATGATGGAAACCATGGAG10420.196103155529375No Hit
ACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCCAG9550.17972985943431202No Hit
CCTTTATACAGAAGGCCCCTGGTCTTTTTTCAAGCCAGTCCCCCACCATA9330.1755894857091237No Hit
CCATGTTGACTGTGTCTTTGATGTTTTCAGCCCAAGTGGTGCGGGGTCTG8630.16241556931079715No Hit
GATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCACAGTG7950.14961805052385138No Hit
ATACAGGACATGGGCCTACCATGGGAGCTACGAAGCCCCCACGCAAGGAT7360.138514320988119No Hit
GAGTAACACTTGTCCCATCTTCTTCTCCACCTGGGGGTCTATTGTCATTG7210.13569133890276333No Hit
CTGTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTG6610.12439941056134059No Hit
GTCCTGTATGGGTGGTTTTCATCAAGAAACCATGTTTCTGCATGTTCATT6170.1161186631109639No Hit
GCCCAAGTGGTGCGGGGTCTGTGCCCTATAAGGGATCCACACCATAAGTC6150.11574226549958314No Hit
ATCTAATGGGAAGGAGAGAAGAAGGAGCAACCATGGGATTCTCAATGGAC5830.10971990371749102No Hit
CGTTGTACACACAGCTGTGACACTCTCCTCTCAGGTGGTGTTCTCTCTCC5660.10652052402075456No Hit
CTCTATTCCACACCATGAGCATGTCCTCAGTGGTCATCCATTCTCCCTTT5610.10557952999230268No Hit
CTTCAGTGATACCTGACTTTTTATAAGAGTAGAACTCCAGGGCCGACATC5320.10012176462728167No Hit
TCTCTACACACTTCAGTGATACCTGACTTTTTATAAGAGTAGAACTCCAG5320.10012176462728167No Hit
GTCCTGAAGTGGAAGAGACACGAACACTCAGAGTGCTCTCTATGGTGGGG5320.10012176462728167No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGTC100.0072371583144.006594
CGATAAG100.0072371583144.006591
TACGTCC203.9722028E-4108.004945
TCCGGGT851.3764293E-859.2968332
TGTGGAC9400.056.683453
GTGGACT3250.053.1716654
CTCCGGG1057.28196E-848.0021931
ATCTAAT3100.046.4537431
AGCGTGC657.5860234E-444.309723
GCGTGCA657.5860234E-444.309724
TCTAATG3300.043.6383632
CTAATGG3350.040.837693
CGTGCAT750.001533703438.4017565
CATCTAA2650.038.039481
TGGACTC1752.593879E-937.0302665
GAACGTG1001.4479508E-436.0016486
TAGGACC5000.036.0016484
CGTCAGA800.002105617436.0016482
TAATGGG3850.035.5340924
TCCCCAC4350.034.7602121
Produced by FastQC (version 0.11.2)