FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_infko_f-black_liver_pcr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_infko_f-black_liver_pcr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878676
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTAAGGACTTCCTGTAGCTACTCCCAGGCTTACCATTCTGCAGTTGTAC51130.5818982195940255No Hit
GAAGAGAGTATGGGCATCAGAAAAGAGTTGATCAGTCTTTCTTTACAAGC31920.3632738347240621No Hit
GTGCGTGGCTAAGAAAGGACACAGAAGAGAGTATGGGCATCAGAAAAGAG29350.3340252834947125No Hit
GTGCGTGGCTAAGGACTTCCTGTAGCTACTCCCAGGCTTACCATTCTGCA29100.33118009368641No Hit
CCCCCAGACTGAGTGGGGGCTCTGTAAGAGCAGGGCTGAGGAGCAGGGAC27690.31513322316758396No Hit
GTCAGTGCGTGGCTAAGAAAGGACACAGAAGAGAGTATGGGCATCAGAAA27260.31023949669730366No Hit
GTGCGTGGCCAGCCACTTAGAATTATGTGGCTCTCTACTACCTTGGATCA19060.21691727098498195No Hit
GTGCGTGGGCGGGGCCCAAAGTTCCATCCCGGCCAGGGAGTCTCCCAAGG18780.21373065839968317No Hit
GTCAGTGCGTGGCTAAGGACTTCCTGTAGCTACTCCCAGGCTTACCATTC17830.20291893712813372No Hit
GGTAATACAGATCTGAAAAGTCAAGGCCTGTCCTTGGTTCACAGTCAAGT16510.18789633494029653No Hit
TCCTGTAGCTACTCCCAGGCTTACCATTCTGCAGTTGTACTCATTCCATG16240.18482352994732984No Hit
ATCTAAGCTTGTCCATTTTTAGGCGGCATTTCAAGTGGCCAGAGAACAGC15920.18118168699270265No Hit
GTATTACCTGACCATGAACAATAAGCATTGGTTGGTGCACAAAGAGTGGT15290.17401180867578037No Hit
GTCTTTCTTTACAAGCTAGATGGAGAGAAACATGAAGACAGCAAAGTTGC15220.17321515552945568No Hit
AGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTAATTTGGATTTG14510.1651348164738766No Hit
ATGCTGGATGAGGGAGTGGAACCAGATGATGTCGATTGCTGGTGCAACAC14220.1618343962962457No Hit
CCTGTAGCTACTCCCAGGCTTACCATTCTGCAGTTGTACTCATTCCATGG13980.1591030140802753No Hit
GTGCGTGGCCGGGTAGCTCCATCACGCGTGCGCGCACCTCGCGGCCTGAC13310.15147790539402464No Hit
GTGCGTGGCGTGGCCCGCATCTCGGATGCCCTGCTGCAGCTCACCTGCAT13230.15056744465536784No Hit
GAGTATGGGCATCAGAAAAGAGTTGATCAGTCTTTCTTTACAAGCTAGAT13050.14851890799339004No Hit
GCTAAGAAAGGACACAGAAGAGAGTATGGGCATCAGAAAAGAGTTGATCA13000.14794987003172955No Hit
GTCATAGGAGTTGGGGACAAGAAAATCACCCACCACTGGCATAGGAGTGG12840.14612894855441597No Hit
GTCAGTGCGTGGCCAGCCACTTAGAATTATGTGGCTCTCTACTACCTTGG12820.14590133336975175No Hit
GTATATGACTTTTTGGCTCGTCGAGCTTCCCAAAAGCCAGGCAATGGCAA12690.14442183466943445No Hit
CTCATACAGTCTCCTGTTTCTCGAGCTCTCTGCAATTTCCTCATGCAACT12520.14248710559978878No Hit
CTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTA12390.14100760689947148No Hit
GTCAGTGCGTGGCCGGGTAGCTCCATCACGCGTGCGCGCACCTCGCGGCC12220.13907287782982578No Hit
GTCAGTGCGTGGGCGGGGCCCAAAGTTCCATCCCGGCCAGGGAGTCTCCC11870.13508961209820228No Hit
TTCATATCCTGTATCATTGACAATCATCCCGCTATGCTGGGAGCCATGCA11850.1348619969135381No Hit
GAAAAGAGTTGATCAGTCTTTCTTTACAAGCTAGATGGAGAGAAACATGA11760.1338377285825492No Hit
CTCCCAGGCTTACCATTCTGCAGTTGTACTCATTCCATGGCTCCAGTTCC11700.1331548830285566No Hit
ACACAGAAGAGAGTATGGGCATCAGAAAAGAGTTGATCAGTCTTTCTTTA11640.13247203747456401No Hit
GTAGTATGCACTCCCGCGTCTAGTGATCTCTGCTGCCATGGCTGTAGTCA11440.130195885627922No Hit
GTGCGTGGCCACCCCCAGACTGAGTGGGGGCTCTGTAAGAGCAGGGCTGA11430.1300820780355899No Hit
CTCTACTACCTTGGATCATGAACCCCGAGACCTTCCTCTGTGGGGCCCGT11270.12826115655827633No Hit
GTCTTTAGCTGGTCCACTCAGCCACCACAACACCAGAGAGGGTTACAGAA11080.1260988123039664No Hit
TAATAAAGAAGTTCAAGAAAGATCTTGCTGCCATGTTGAGAATAATCAAT10940.12450550601131702No Hit
GAATATGACACGCCCTTCAATCTAAGCTTGTCCATTTTTAGGCGGCATTT10720.12200173898001083No Hit
GTTCGAGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTAATTTGG10720.12200173898001083No Hit
TCCTTGTAGAGTGAGAAGGGAGCGTCACGGCTCTTCTAGATCGCCGTGCC10560.12018081750269724No Hit
TTCCATGGCTCCAGTTCCAATCTAGGTGCAATTAAACTTTCCTCTCATAC10550.12006700991036513No Hit
GTATGGGCATCAGAAAAGAGTTGATCAGTCTTTCTTTACAAGCTAGATGG10480.11927035676404044No Hit
CTCAATATGTCTGCAAAAGAACATTAGTGGACAGAGGTTGGGGAAACGGT10450.11892893398704416No Hit
GAGTTATGAGTGCCCTATGCTGGATGAGGGAGTGGAACCAGATGATGTCG10420.11858751121004785No Hit
TCTTAGAACACGTAAACTTGGCACATGTCACCAAGCTCCCTTTGCCAAAA10350.11779085806372315No Hit
CTACTACCTTGGATCATGAACCCCGAGACCTTCCTCTGTGGGGCCCGTTT10290.11710801250973055No Hit
GTCTAAGATATTTATACTCCCCACTGTGGCCAGCAAACATAACTGGTGTG10070.11460424547842436No Hit
GTATCAACAGGTTTAATTTGGATTTGGAAACGAGAGTTTCTGGTCATGAA9950.11323855437043917No Hit
TCCTAGAGGAGAATGGAGTTCAACTGACAGTTGTTGTGGGATCTGTAAAA9930.11301093918577496No Hit
GTGCGTGGCACGGCCATTGCTCGAATTGCCAACCAGGCCAAAGCAAATCC9900.11266951640877866No Hit
TTAGTGGACAGAGGTTGGGGAAACGGTTGTGGACTTTTTGGCAAAGGGAG9740.11084859493146508No Hit
CTCCAAGAGAGAAGTGGTCCATATGATCGGTTGACCCCGCTGTCACCATT9470.10777578993849837No Hit
TCCGTACACAACCCAAGTTGATGTCGTGTTGCACCAGCAATCGACATCAT9370.10663771401517737No Hit
GGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTACCACATTGGGAG9330.10618248364584898No Hit
GTTAGTAACATGGCCGAGGTAAGATCCTATTGCTACGAGGCATCGATATC9260.10538583049952428No Hit
GCTAAAGACTTGGGTATGATAAGATCACTTTCTTCTACTCCATCTGTCCA9120.10379252420687488No Hit
CTTCTAGATCGCCGTGCCTCACCTTTTTTGTGATGACAGGTTCCGTACAC9050.10299587106055018No Hit
ATATCGGACATGGCTTCGGACAGTCGTTGCCCAACACAAGGTGAGGCCTA9040.10288206346821809No Hit
CATATTGAGTGTCTGATTGCTTGTCAAGGTAGGCCTCACCTTGTGTTGGG9020.1026544482835539No Hit
TCAGAAAAGAGTTGATCAGTCTTTCTTTACAAGCTAGATGGAGAGAAACA9010.10254064069122179No Hit
AGAAAGGACACAGAAGAGAGTATGGGCATCAGAAAAGAGTTGATCAGTCT8950.1018577951372292No Hit
GTACAAGTACCATCCTGACTCCCCCCGCAGATTGGCAGCAGCAGTCAAGC8940.1017439875448971No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGCGT16950.0109.565064
GTCAGTG17050.0109.3570861
CAGTGCG17150.0108.7070543
TTCGAGT2350.094.9599842
TCGAGTC2350.094.954583
GTTCGAG2450.091.089251
CGAGTCT2750.081.1430054
TTGTCAG554.6142304E-878.534431
TGCGTGG35700.073.598922
GCGACAG406.899914E-571.98991
CGACAGT502.4047931E-671.989891
CCGGGTA3400.067.7320569
CGTGGCT12950.066.701194
GTGGCTA13050.065.638495
CCAGACT7750.064.086934
ACTGAGT7900.062.8557788
GCTAAGG13350.062.5530171
CCCAGAC7950.062.4746863
GTGCGTG42550.062.2615281
CTAAGGA13450.062.0844042
Produced by FastQC (version 0.11.2)