FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_infar_m-black_blood_pcr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_infar_m-black_blood_pcr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences586698
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCA22730.3874224899351966No Hit
TATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTG12750.21731793870100122No Hit
GTATAAAGGTGCTGTGCCCATACACCAGCACTATGATGGAAACCATGGAG10670.18186528674036728No Hit
ACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCCAG9460.16124138824403697No Hit
CCTTTATACAGAAGGCCCCTGGTCTTTTTTCAAGCCAGTCCCCCACCATA8740.14896931641150982No Hit
GATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCACAGTG7070.12050492757773165No Hit
GGTGTGGACATCCCCAAGGTTAAGTGGTCTGCCCCGTATAAGGTCAGGAG6500.11078953737698101No Hit
ATACAGGACATGGGCCTACCATGGGAGCTACGAAGCCCCCACGCAAGGAT6370.10857374662944139No Hit
GTCCTGTATGGGTGGTTTTCATCAAGAAACCATGTTTCTGCATGTTCATT6240.10635795588190176No Hit
CTGTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTG6210.10584661955554647No Hit
GAGTAACACTTGTCCCATCTTCTTCTCCACCTGGGGGTCTATTGTCATTG6080.10363082880800684No Hit
GGTGTGGACCCTTCCTCACCCAAGTAGCGGACTTGGGTGGATAGATAGTC5930.10107414717623037No Hit
CCATAAGTCCTCCCTTTTTCCTAGATAGGGAATGTCTGTCCATTTTGTTA5870.10005147452351977No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG406.3839616E-789.970325
CATCTAA2650.081.50341
ATCTAAT4800.074.994451
CCGTGCG502.4065012E-671.9762659
ACCGTGC502.4065012E-671.9762658
TGTGGAC17500.071.564973
GGACCGT451.237041E-463.9789056
GTGGACT4950.056.708574
TCTAATG6400.056.2362482
CGATGCG400.006225662353.9821939
GACTCGA553.333761E-452.3508382
CTAATGG7150.050.333053
TCCGGGT2300.050.074712
TAATGGG7050.050.0260544
GTGGACC7050.047.9841774
ACTCGAC605.123323E-447.9841773
TCGACAG752.660914E-547.9841775
GGTGTGG30400.044.996671
CTCCGGG2600.044.3044171
CGCCTTA1151.4939906E-743.8116389
Produced by FastQC (version 0.11.2)