FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_female_transmission_liver_pcr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_female_transmission_liver_pcr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences404822
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCA19480.48119914431527927No Hit
TATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTG12100.2988967990870061No Hit
ACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCCAG8360.20651051573284063No Hit
GTATAAAGGTGCTGTGCCCATACACCAGCACTATGATGGAAACCATGGAG8230.2032992278087653No Hit
CCTTTATACAGAAGGCCCCTGGTCTTTTTTCAAGCCAGTCCCCCACCATA5870.14500200087939885No Hit
GGTGTGGACCCTTCCTCACCCAAGTAGCGGACTTGGGTGGATAGATAGTC5860.14475497873139306No Hit
GTCCTGTATGGGTGGTTTTCATCAAGAAACCATGTTTCTGCATGTTCATT5600.13833240288324253No Hit
GATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCACAGTG5520.13635622569919617No Hit
ATCTATGCCGCATTGACAACTCTCATCACCCCAGCTGTCCAACATGCGGT5430.13413302636714408No Hit
CTGTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTG5410.1336389820711325No Hit
ATACAGGACATGGGCCTACCATGGGAGCTACGAAGCCCCCACGCAAGGAT5360.13240387133110354No Hit
ACCATGGGATTCTCAATGGACATTGATCTGCGGCCAGCCTCCGCCTGGGC5260.12993364985104566No Hit
ATTCCACACCATGAGCATGTCCTCAGTGGTCATCCATTCTCCCTTTCCAT4990.12326405185488931No Hit
GAGTAACACTTGTCCCATCTTCTTCTCCACCTGGGGGTCTATTGTCATTG4560.11264209949064033No Hit
TCTCTACACACTTCAGTGATACCTGACTTTTTATAAGAGTAGAACTCCAG4230.10449036860644925No Hit
ATCTAATGGGAAGGAGAGAAGAAGGAGCAACCATGGGATTCTCAATGGAC4200.10374930216243189No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATGCG301.1477823E-7119.984269
TGGACGA451.2842993E-679.98955
ATCTAAT2350.067.403741
TGTGGAC11950.062.6528663
CATCTAA1051.6370905E-1161.713811
ACGATGC607.08856E-659.992138
GACGATG607.08856E-659.992137
TATCAAG400.006219387553.9929167
CTAGGCG400.006219387553.9929163
CTAATGG3000.052.793073
GGACGAT701.7663631E-551.4218256
TAATGGG2950.051.247514
GGACAAT859.355663E-750.816866
TCCGGGT1501.0913936E-1147.9937022
GTATAGA7850.046.776711
TAGATAA8300.044.2351534
TCTAATG3750.044.1542052
AGATAAG9000.040.7946435
AATGGGA4000.039.5948035
TATAGAT9150.039.33912
Produced by FastQC (version 0.11.2)