FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_female_transmission_blood_pcr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_female_transmission_blood_pcr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences254095
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCA8550.3364883212971526No Hit
TATCTATACAGTGACGAGAAACGCTGGCCTGGTTAAGAGACGTGGAGGTG4760.1873315098683563No Hit
ACATGTACTTGATCCCAGGCCTACAAGCGGCAGCAGCGCGTGCTGCCCAG3530.1389244180326256No Hit
GTATAAAGGTGCTGTGCCCATACACCAGCACTATGATGGAAACCATGGAG3130.1231822743462091No Hit
GATAAGGGAAGCTCCTGCCAGATAGCTTCCTCTGAAGATGTTGCACAGTG2680.10547236269899053No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCTAA1350.064.010511
TGTGGAC6750.063.9979023
ATCTAAT1850.062.280491
TGGACCG400.006219153353.9875955
GTGGACT1650.052.3516044
GGGTGTG4500.049.6081431
GTATAGA3750.048.007881
TCTAATG2550.047.998422
TATAGAT3700.046.7011682
TATACCT952.011835E-645.4632345
AGATAAG3650.045.359445
CTAATGG2550.045.1749843
TAGATAA4100.042.1366584
TGGACTC1054.004887E-641.1334085
TACCTTG1054.021187E-641.109127
TAATGGG3000.038.391184
GGTGTGG11700.038.1601071
CCGGGTG951.0683894E-437.900961
ATATACC1157.47979E-637.5565874
GTGTGGA12700.035.1484532
Produced by FastQC (version 0.11.2)