FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_female_transmission_blood_pcr2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_female_transmission_blood_pcr2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences357331
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCACTGGAGATGGTGAGAGGCAGGAGTTACTCTAATGAACATATGTGTCC25080.7018702547497978No Hit
GCACTGGAGATGGAGCGTGCACCACTTGGCTCTGCTCTGCTTCTTATGAA19150.535917678566931No Hit
GCACTGGAGATGGCTACTGCTATGAGTAACACTTGTCCCATCTTCTTCTC16000.4477641178627099No Hit
GCACTGGAGATGGAAGAGAGAATCTCAGGTGCAGAAGATTCCATAGAGAA10890.3047594527203069No Hit
GGAGATGGAGCGTGCACCACTTGGCTCTGCTCTGCTTCTTATGAAGACAT6450.18050491001340493No Hit
GTATAACATCTGTCCAGGCTCTTCTGGCTTTCATAGTCTCTGGTGAAAAA5000.13992628683209685No Hit
GCACTGGAGATGGCTGGCACAGGCCATCAGACTCCCTAGAAAGCAGCAGT4740.13265011991682782No Hit
TTTGTTGTTCTGATTATTATGTGTCGGGAGGAATTTCTTTTCTGGTCCAG4340.12145601697026007No Hit
CAATAAGAAGACCAAACGTACGGATAATAGGAGTGGATGAGAATGAAGAT4270.1194970489546107No Hit
GCACTGGAGATGGGAAACAAGGACAGATATAAATACAGAAAGCATCTATT4140.1158589654969762No Hit
CTATTAATGTGGTAGGACTACTGTTACTCACAAGGAGTGGGAAGCGGAGC4000.11194102946567748No Hit
GAGATGGAGCGTGCACCACTTGGCTCTGCTCTGCTTCTTATGAAGACATG3720.10410515740308006No Hit
CTCCCTCTATGATAATTGAGAGTTTGGCTGGGTATAGTAGCCTGGGCTGC3670.10270589453475909No Hit
GGTCTGAACAGACCCTAGCATCCATCCATCTCGGTTTGAGCACTCTCTTC3630.1015864842401023No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATCG100.007241982143.967945
ACTGGAG20600.0109.738513
TGGAGAT22600.0100.968675
CTGGAGA23300.098.553174
GATGGCG651.6370905E-1188.5956659
GCACTGG26500.085.589821
GGAGATG28250.083.577866
CACTGGA27200.083.3754652
AGATAAG1600.072.004131
GAGATGG31800.071.078527
GTTACTA502.0777847E-457.6033021
GGCTAAG400.00621415554.0030981
ACTATCC400.00622104153.987984
GATGGGA2550.053.6351179
AGATGGT10500.049.360448
TAGACAT450.00990982347.989325
CTGTGCC4700.045.9536483
AGATGGG6600.044.717328
AGATGGA22600.042.999288
GATGGGG3600.037.9915439
Produced by FastQC (version 0.11.2)