FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_demux_negative_control_pcr3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_demux_negative_control_pcr3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11
Sequences flagged as poor quality0
Sequence length150
%GC38

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGGATGGGATAGACTCAAACGAATGGCGGTCAGTGGAGATGACTGCGTT19.090909090909092No Hit
ATTCTTTAGATATCACATTAAGCCATAACATAAAAAAAAAAAAAGCGCTC19.090909090909092No Hit
TAATTGGACATTAAACAGAAAACATCCGCCAGAAACTGCAATGGCCAACA19.090909090909092No Hit
CTGTATGACAATTGTAATGGCTAACTTAATATTACAAATTGGAAACATAA19.090909090909092No Hit
ATGTAAATCAGACAAAAATTTTCCTAAAATTCAATAAGCACAAAATTACA19.090909090909092No Hit
GCTTGCCCTGTATGCAGCCGTTCGGCTGGAATACATCGTCAAAGTCTCAA19.090909090909092No Hit
AATCCGGGAGACAAAATAACATAACAAGCAACTTGAAAAATATTTGAAAC19.090909090909092No Hit
TATCCATGTTGTTCGGGTCCCCATTCCCATTTAGGGCATTTTGGACAAAG19.090909090909092No Hit
CTTATTGTTCATGGTAAGGTAATACAGATCTGAAAAGTCAAGGCCTGTCC19.090909090909092No Hit
AAACAGAACTGATTAAAAAACTGACCACTCCTAATAATTAAAAAAAAAAC19.090909090909092No Hit
GAGAAAAAGAACTAACACGCTAAGAATAGATGTGAATGAGACGCTAGTTT19.090909090909092No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)