FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_demux_negative_control_pcr2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_demux_negative_control_pcr2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50
Sequences flagged as poor quality0
Sequence length150
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACATACCACGATCCTGCAGCAAAAGGTATAGCTATTGGGTTCATGCCAC12.0No Hit
CTCTAGTAGATGCCTAAAACCAGTCATACTTGATGGTGAGAGAGTCATCT12.0No Hit
CATCCACACCATGTGGCACGTTACAAAACGAGCCGCACTGAGGAGCGGTG12.0No Hit
TGTTCAAACCTAACCACACTAGCAGCGTGCCTATGAGGATCTGTGAGAAC12.0No Hit
GCTTAGGGGCATCGGGAAAGCTGGGTGGTGCCTCCGCCAGTGGTCGGGGA12.0No Hit
GATCATAACTCCCATTCATGGCAATAATGCTCTAAATATCAAAAAAACTA12.0No Hit
GGCACGGCCATTGCAATAATTGCAAACAAGGCCAAAGCAAATCCAGTAAT12.0No Hit
GCTTTGGCCTGGTTGGCAATTCGAGCAATGGCCGTGACACGCACTGACAA12.0No Hit
GACAGCGATCACTCCGGCGGCAACAGTGCAACAATCCTCATAACATCCCC12.0No Hit
GGTAAATAGGTGTCCGATAAACAAAATAAGACGGTTGAATGTGAGGAGCG12.0No Hit
GCCCTGTAGATCGCCACAACAAGAAGTAAACCTTCTACCATCTTCATCTC12.0No Hit
GTACACTCCAACAGTTGTCTATCATGTCTTCACATCATTTCGAGCAAGCA12.0No Hit
CTCTTGGAGTGATTGTGATTCTACACATGGTGCAGGAGGGGTTGAAGACA12.0No Hit
CACGGCAATTTAAAGGGAGTGGGAAGACGAAAAGTCCATTTGGACTGGCA12.0No Hit
AATGATGAAAGCCACATCAACCGAACATATGGACACCTTCTCTGTTGGAG12.0No Hit
TGATGGACCGGGGCAAGCGAACAGTTGACATAGAGTTGGTCGCGGCGACG12.0No Hit
GAGAACGGCACAGGACCTTGTATGGCTGGAAAGAACAAAAAATGACATAG12.0No Hit
TGCAACTTCCTTGTAGAGTGAGAAGGGAGCGTCACGGCTCTTCTAGATCG12.0No Hit
GAACAGTCACAGTGGAGGTGCAGTATGCAGGGACAGATGGACCCTGCAAG12.0No Hit
GCCTCCATTACTCCCAAAGCCGCTCCTCTTTTTCCAGCGGCGAATTCTTT12.0No Hit
CTTCTAGGTATTTATTTCTTCTCTCATCAAAAGCAGAAAGCACCATCCAC12.0No Hit
GATATATGGAGGATTTTCAATTAGTGACACTAAAAATAAAACACAACTGA12.0No Hit
GGGAGATAGATCCATTCTTTGTGTTCAAACCTAACCACACTAGCAGCGTG12.0No Hit
TAAACTGAAGTCCTACCACATTAATAGGGTCAACTGCCCTCACAGATGTA12.0No Hit
GCTATACCTTTTGCTGCAGGAGCGTGGTATGTGTATGTGAAGCCTGGGAA12.0No Hit
GTCCTTAAGCATCTGCCTTCAGCTCAGGTCATGATCCCAGGGTCCTGGAA12.0No Hit
TGCTGGTCTCCTTCATTTTCAGAGCCAATTGGACACCCCGTGAGAGCATG12.0No Hit
CCAGAGAGCAACTTAGTGAGGTCAATGGTGACAGCGGGGTCAACCGATCA12.0No Hit
GCTTAAACTCTCCCTCAATGGCGGCTACCTTATCGGCCTCAGGCCGATAG12.0No Hit
GGCTTCCTGAAACCTCTCTGTCATGTGTCCTGGCAGTGTTCCCAGGGCAT12.0No Hit
GTGATACACTTAAGGAGACACAAGCTGACATAGGAGAATTATGAAAATAT12.0No Hit
AATGTAAGAATCCCCAAATGGTGGGTCAAGCTCCAACATCATCTTTGAGT12.0No Hit
ACCTTGATGTTGGCCAAGAAGTTCGTAAAACAAGTCAGATATCGAACTCT12.0No Hit
GAAGACAGTCACGAAAAACAAGACACTAGCAGAGAGACTAGAGCAATTGA12.0No Hit
ATCCTAGACAAATGTGGAAGAGTGATAGGACTATATGGCAATGGGGTTGT12.0No Hit
CTCCCTCTTGCATGACTCCCACTCCAACCTGTGTTGAACCTAGCAGTCTG12.0No Hit
GGTTCCTGCCTATCCGTCCTCTCCTCTGAGCAGCACTAGCATGCGTGACA12.0No Hit
AGTGTGGGCAGTCCCACCTAGATTGAGCTGCTCCTGGACACGTGGGTTAA12.0No Hit
CAAAGTATATCAATTATTGGCAAGTCAACATTCAAAAAAAACACTCACTG12.0No Hit
GTTCACTCCCAATGTGGTAGCAAACGAAATGGCCTTCCCGGCATCGCTCC12.0No Hit
GGGGGGGGCGGGGGGGCGGGGGGGGGGGGGGCGGGGGGGGGGGGCCGCCG12.0No Hit
TGATATGCAAGACATACGGGAAGGTCTCCTCTCTTAATGATTACCACGAA12.0No Hit
CTATTACTCACAAAGAAATGCAATAAGACAAAACCACAGAGAATAGTACA12.0No Hit
GCCTTATAGGGCCTGCTTCCAGTATCTGCTCGCATGATCACGGCGAGGTT12.0No Hit
TTGCTGGTCTCCTTCATTTTCAGAGCCAATTGGACAACCCGTGAGAGCAT12.0No Hit
GCAATAGGATCTTACCTCGGCCATGTTACTAACCGTCGTCGTGACAAACT12.0No Hit
AGGTATAGCTATTGGGTTCATGCCACAGATGGCCATCAGGACCACCTTGA12.0No Hit
TCAAAACGGTTCATTACACAGATGGCCTTCAGGAACCACTTGAGTAATAG12.0No Hit
TAGCGGGATAAAATACAATAATAAAGTATAAGAAACTTACGAAAAAAAAG12.0No Hit
CTGCTAGCCCTGGCTTCGTGTCTTTTGAAAACTGCGATCTCTGCTCTTGA12.0No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTCA50.0144.05
GTCACGA50.0144.08
CAGTCAC50.0144.06
GACAGTC50.0144.04
TCACGAA50.0144.09
AAGACAG50.0144.02
AGACAGT50.0144.03
AGTCACG50.0144.07
GAAGACA100.072.01
Produced by FastQC (version 0.11.2)