FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_demux_negative_control_pcr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_demux_negative_control_pcr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences73
Sequences flagged as poor quality0
Sequence length150
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCCAAGTACATGTAGTATGCACTCCCGCGTCTAGTGATCTCTGCTGCCA11.36986301369863No Hit
GTGTCTGGCTTAAGGTCAGAGAAGATTACTCATTAGAATGTGACCCAGCC11.36986301369863No Hit
TTAATCGAGTTCCGCTTGTATACTACAGGTAAACCTGGGTCCCGGGCCAT11.36986301369863No Hit
GTGCATGGACATTACTCAAAACGGATTTCAAGCACTTCACTGTGCCATGG11.36986301369863No Hit
TCTTAGAACACGTAAACTTGGCACATGTCACCAAGCTCCCTTTGCCAAAA11.36986301369863No Hit
GATCCTATTGCTACGAGGCATCGATATAGGACATGGCTTCGGACAGTCGT11.36986301369863No Hit
GTTCAGAGCAGATTACTCATTAGAATGTGACCCAGCCGTCATAGGAAAAG11.36986301369863No Hit
AACACACCTGGCCCCAGTACAGACGACCTGAGTCAATGTGTTCCCTTTCA11.36986301369863No Hit
ATCCTGTATCATTGACAATCATAACTCTATGCTGGGAGCCATGCACTGAT11.36986301369863No Hit
TAGTAACATGGCCGAGGTAAGATCCTATTGCTACGAGGCATCGATATCGG11.36986301369863No Hit
CAATGGTGACAGCGGGGTCAACCGATCATATGGACCACTTCTCTCTTGGA11.36986301369863No Hit
TTGATTGTCTGATTGCTTGTCAAGGTAGGCCTCACCTTGTGTTGGGCAAC11.36986301369863No Hit
CTTATCTATACATAACAGAGAAACTCAAACCAGGAAAAGAGAAGTGGCAC11.36986301369863No Hit
TCTCGCTGGAGCTCTAGAGGCTGAGATGGATGGTGCAAAGGGAAAGCTGT11.36986301369863No Hit
GGCTTCGGACAGTCGTTGCCAAACACAAGGTGAGGCCTACCTTGACAAGC11.36986301369863No Hit
GCAATAGAGACTTCTTGGAGGGCATGTCAGGTGGGACCTGGGTTGATGTT11.36986301369863No Hit
GCATGGAACAGTCACAGTGGAGGTGCAGTATGCAGGGACAGATGGACCCT11.36986301369863No Hit
ATATACATTTATCAACAATTAACAAGAGCTTAGTGTATTCTATTGATTAA11.36986301369863No Hit
TCCATGCTCTGTGCTCAAGCGGACATTCCTTCAGTGTGTCACCGTCGACA11.36986301369863No Hit
AGCATGCCAAGGCAATGGGATGTCATGAAACCACTCTTTGTGCACCAACC11.36986301369863No Hit
TATCTACTCTTATAAAAACTCAGTTATCAATCAAAAGTCTAGATAAAGAT11.36986301369863No Hit
GTGGTTAGGTTTGAACACAAAGAATGGATCTATCTCCCTCACATGCTTGG11.36986301369863No Hit
GGACATTGAGGAATGGTGCTGTAGACAAAGCACAATGCCCCCACTATAGT11.36986301369863No Hit
TATCACGGCTGGCTCACAATCTAATGAGTAATCTTCTCTGACCTTAAGCC11.36986301369863No Hit
GATTTCAAGCTCTTCACTGTGCCATGGCCCTTTCACTTGGGTTCTGTAAC11.36986301369863No Hit
CTGTATCATTGACAATCATCCCGCTATGCTGGGAGCCATGCACTGATAGC11.36986301369863No Hit
TCCTATGACAATGTAAGAATCCCCAAATGGTGGGTCAAGCTCCAACATCA11.36986301369863No Hit
GAACAGCAAAGATCATGCTGCCATCTTGAGAATTATCAGAGCTTGGAAAT11.36986301369863No Hit
TTCCCTGAATAATGCACCAACACTGTGACTGTACCATGAAGTGTTAAAGC11.36986301369863No Hit
CTCCAGATATTCCGGTACAAAGCTCATCCCGTTAATCTTCTTAGAACACG11.36986301369863No Hit
TTACAGAACCCAAGTGAAAGGGACATGGCACAGTGAAGAGCTTGAAATCC11.36986301369863No Hit
GTATTACCTTACAATGAACAATAAGCATTGGTTGTTGCACAAAGAGTGGT11.36986301369863No Hit
TCATTAAAGATGAATACCCCCGTGCCACATCTCGTTACCTTTTTTGAGAA11.36986301369863No Hit
CTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTA11.36986301369863No Hit
GTGGCCTAAAATGCATATCCGATGTTGCTACCACTCCTATGACAGTGGTG11.36986301369863No Hit
AAATAACCGTCGACGAGACAAACACAATGTCAAATGTTTGAATGTCCTTA11.36986301369863No Hit
TTGAGGTGGCTGAGTGGACCAGCTAAAGACATGGGTATGATAAGATCACT11.36986301369863No Hit
TTCCTTCAGTGTGTCACCGTCGACAACAAAACTGTTGTTGGTCTTTGCCG11.36986301369863No Hit
ATCTCAATCAGGTGGGCCCTTTTCAGCCTCCATGTGTCATTCTTTTCACT11.36986301369863No Hit
GCTCCAACATCATCTTTGAGTTCTCAGTGCTTTCAGTAATCACGGGGTTG11.36986301369863No Hit
AACTTAGTGAGGTCAATGGTGACAGCGGGGTCAACCGATCATATGGACCA11.36986301369863No Hit
ATTTTAATTGATGTAAAAAGATGACAGGGATAAACAATGAGACGGAATCT11.36986301369863No Hit
GTGCATAGTACATGTACTTGGAAAGGAGCGATGCAGGGAAGGCCATTTCG11.36986301369863No Hit
ATACAGATCTGAAAAGTCAAGGCCTGTCCTTGGTTCACAGTCAAGTCCTA11.36986301369863No Hit
CCAGCCATGGGGCAGCTCATTCACAGGCACTGGCAATCTTTGTGGACCTC11.36986301369863No Hit
AAACGATAGTGGGGGCATTGTGCATTCCCTACAGCACCATTCCTCAATGA11.36986301369863No Hit
CCCCAACTCCTATGACAAAGTAAGAATCCCCAATTGTTGGGTCAAGCTCC11.36986301369863No Hit
CTTCAACATCATTATAGATGAATACCCCCGTGCCACATCTCGTTTCCTTT11.36986301369863No Hit
AGCTGCCCCATGGCTGGAAAGCCTGGGGGAAATCGTATTATGTTAGGGCG11.36986301369863No Hit
GGGTTTTTTACAGATCCCACAACAACTGTCAGTTGAACTCCATTCTCCTC11.36986301369863No Hit
GGATATGAAACTGACGAAAATAGAGCGAAAGTCGAGGTTACGCCTAATTC11.36986301369863No Hit
CACCTGACATGACCTCCAAGAAGTCTCTATTGCTGCCTCCAATGAAACTG11.36986301369863No Hit
GTCCATGATCTGTACGTGGCACTTGTTCACTACCAATGTGGTAGCAAACG11.36986301369863No Hit
GTTAGTAACATGGCCGAGGTAAGATCCTATTGCATGTCTCTTAAACAAAA11.36986301369863No Hit
GGGAAGAGGGGATCTGTGGGATCTCATCCGTTTCAAGAATGGAAAACATC11.36986301369863No Hit
CCAATGCTTATTGTTCATGGTCAGGTAATACAGATCTGAAAAGTCAAGGC11.36986301369863No Hit
GATGTGGGGTGCTCAGTGGAATTCTCAAAAAAGCAAACGAGATGTTCCAC11.36986301369863No Hit
TCTATAATGATGTTGAAGCCTGGAGGGACCGGTACAAGTACCATACTGAC11.36986301369863No Hit
GCTCTTCTAGATCGCCGTCCCTCACCTTTTTTGTGATAACACGTTCCGTA11.36986301369863No Hit
TTTTTACAGATCCCACAACAACTCTCAGTTAAACTCAATTCTCCTCTAGG11.36986301369863No Hit
ATGGTGGGTCAAGCTCCAACATCATCTTTGAGTTCTCAGTGCTTTCAGTA11.36986301369863No Hit
GGTTTACGTGGAGGAGACATGCGGAACTAGAGGACCATCTCTGAGATCAA11.36986301369863No Hit
GGCCTCGACTTTTCAGATCTGTATTTCCTGACCATGAACAATAAGCATTG11.36986301369863No Hit
GCCTGGGACTTCGGAACAGTCGGGGGTGTGATCAACTCACTGGGTTAGGG11.36986301369863No Hit
ACTAGAGGACCATCTCTGAGATCAACTACTGCAAGTGGAAGGGTCATTGA11.36986301369863No Hit
CTGTTGGCTTGTCCTGTGCCATCACGGTAACGCAGCCTCCATGTTCCAAG11.36986301369863No Hit
CACATAACTCATACTTTAGGCCACCAAGTGCCGCCCAACCAACAGCTGTA11.36986301369863No Hit
CCGTGCCACATCTCGTTTCCTTTTTTGAGAAGTCACATGAGAACCCAACA11.36986301369863No Hit
CATTCTAATGAGTAATCTTCTCTGACCTTAAGCCAGACACTGGTGTGGAA11.36986301369863No Hit
GTTGATCTCAGAGATGGTCCTCTAGTTCCGCATGTCTCCTCCACGTAAAC11.36986301369863No Hit
GAACGACGACGGTTTGCCTCTTGGCGTGGGCATCCTTGAATTCTACCAAT11.36986301369863No Hit
ATTCCCTACAGCACCATTCCTCAATGACCCTTCCACTTGCAGTAGTTGAT11.36986301369863No Hit
CTGAAACACTGCATGGAACAGTCACAGTGGAGGATGTCTCTTATACACAT11.36986301369863No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGAAC50.0144.03
TAGAACA50.0144.04
ACACGTA50.0144.08
CACGTAA50.0144.09
CTTAGAA50.0144.02
AGAACAC50.0144.05
GAACACG50.0144.06
TCTTAGA50.0144.01
AACACGT50.0144.07
Produced by FastQC (version 0.11.2)