FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_cal-09_cb10-14_p2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_cal-09_cb10-14_p2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906686
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA55090.6075973379979398No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG49360.5444001561731404No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA41150.45385061642067925No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG24760.273082412213269No Hit
GTTGGTTCTATTGGAAAATGAAAGAACTTTGGACTACCACGATTCAAATG23570.2599576920786248No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT22190.244737428393071No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG19200.21176019040770452No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT18680.20602501858416256No Hit
CCGTGCCCAGTGAGCGAGGACTGCAGCGTAGACGCTTTGTCCAAAATGCC18660.20580443505248786No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA18020.19874576203889768No Hit
GCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGT17820.19653992672215076No Hit
GGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGGATGGGAACAGT17060.18815775251851247No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA15960.1760256582764044No Hit
GAGGAGGGAGCAATAGTTGGAGAAATTTCACCATTACCTTCTCTTCCAGG15630.17238603000377198No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC15220.16786406760444078No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT14920.1645553146293204No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC14350.1582686839765917No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC14040.15484963923563394No Hit
CTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAACAGGCAGCG13860.15286438745056172No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC13380.1475703826903691No Hit
ATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTGTTCCTG13100.1444822132469234No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT12980.14315871205687525No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA12930.14260725322768852No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT12720.14029112614510425No Hit
GAAAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAATGGGAG12510.13797499906252No Hit
GGCTATGGAACAGATGGCTGGATCGAGTGAACAGGCAGCGGAGGCCATGG12490.1377544155308453No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT12380.1365412061066345No Hit
GATTACTGATCGTCTTTTTTTCAAATGTATTTATCGTCGCTTTAAATACG12320.1358794555116104No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT12150.13400449549237553No Hit
AAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACCATCGT12040.1327912860681647No Hit
GCGGAGGCCATGGAGGTTGCTAATCAGACTAGGCAGATGGTACATGCAAT11730.12937224132720698No Hit
AGTAGAAACAAGGTAGTTTTTTACTCTAGCTCTATGTTGACAAAATGACC11680.12882078249802026No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT11570.12760757307380946No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT11560.1274972813079721No Hit
GGTCTGAAAGATGACCTTCTTGAAAATTTGCAGGCCTACCAGAAGCGAAT11410.1258429048204119No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC11380.1255120295228999No Hit
CATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAG11290.12451940363036376No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT10740.11845335650930973No Hit
GTGCTAGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGT10570.11657839649007484No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG10440.11514460353418934No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG10100.11139468349571958No Hit
AGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCTGCTG10090.11128439172988222No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC10060.11095351643237018No Hit
GGTCTAGTGTGTGCCACTTGTGAACAGATTGCTGATTCACAGCATCGGTC9930.10951972347648468No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA9830.10841680581811122No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT9730.10731388815973777No Hit
GGATCGAGTGAACAGGCAGCGGAGGCCATGGAGGTTGCTAATCAGACTAG9690.1068727210963884No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC9620.10610067873552698No Hit
CCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCATGTCAAAG9590.10576980343801493No Hit
CCTCTCGTCATTGCAGCAAATATCATTGGGATCTTGCACCTGATATTGTG9390.10356396812126802No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG9370.1033433845895933No Hit
GCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAAAC9320.10279192576040659No Hit
GTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTAGTG9230.10179929986787048No Hit
AGCCAGCACCATTCTGTTTTCATGCCTGATTAGTGGATTGGTGGTAGTAG9230.10179929986787048No Hit
CTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATCATCCCGTCAGGCCC9230.10179929986787048No Hit
TCCTAGCTCCAGTGCTGGTCTGAAAGATGACCTTCTTGAAAATTTGCAGG9180.10124784103868374No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG100.0072433976143.970665
GAGCGAA3400.0124.9295041
GCAGGTA11600.089.981679
GAGCAAA33100.081.7808761
GCAGGGT18100.080.734939
AAGCAGG43650.071.408137
AGCGAAA6950.070.439461
AGCAAAA40950.066.100072
GCGAAAG7450.065.708362
AGCAGGG24700.065.282248
GCAAAAG41700.064.5623863
CGAAAGC7500.064.306893
CACCTAC451.2366327E-463.9869583
AGTAGAA8700.060.4081461
AGGTCGT1450.059.574076
CAGTAGG13750.057.0647359
GTAGAAA10900.056.8020321
AGCAGGT22200.054.799648
CAGCAGT13800.054.7714466
GGTCGTT1450.054.6095667
Produced by FastQC (version 0.11.2)