FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_blood_ifn--_red_needle_f12_pcr1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_blood_ifn--_red_needle_f12_pcr1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences906739
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACAT142051.5666029585139716No Hit
ATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTC133591.4733015785137729No Hit
CTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAAC96371.0628196206405591No Hit
GCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATG63940.7051643306397982No Hit
GTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAG61590.679247280639743No Hit
ACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTG60860.6711964523418535No Hit
TGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTG57910.6386622831928482No Hit
CATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGC54510.6011652746821301No Hit
GATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGT54150.59719500319276No Hit
GTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGC44310.48867424914997587No Hit
GCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCA40930.4513978112775562No Hit
CGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTG38010.4191944980859983No Hit
GTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGG36750.40529854787320274No Hit
TGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCA32800.3617358468092803No Hit
CACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACA24710.2725150236176011No Hit
GCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGAC23800.26247905957502654No Hit
GTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACG23100.25475908723458457No Hit
ATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCC22740.25078881574521444No Hit
GCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCA21630.2385471453196565No Hit
GGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGAT20200.22277634468132507No Hit
TTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGT18440.20336612851107097No Hit
CAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCG18070.19928557170255165No Hit
CGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGA16700.18417648297911526No Hit
GTATTACCTGACCATGAACAATAAGCATTGGTTGGTGCACAAAGAGTGGT15690.173037665745049No Hit
ATGCTGGATGAGGGAGTGGAACCAGATGATGTCGATTGCTGGTGCAACAC15500.17094224468121477No Hit
ACTGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAA14830.16355312829822033No Hit
GGTAATACAGATCTGAAAAGTCAAGGCCTGTCCTTGGTTCACAGTCAAGT14760.16278113106417613No Hit
TGACAACATCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACA13930.15362744957479493No Hit
GGTTTAAGGAGACCAATAGAAACTGGGCTTGTCGAGACAGAGAAGACTCT13760.15175259914925907No Hit
CTACATGTACTTGGATAGGAGCGATGCCGGGAAGGCCATTTCGTTTGCTA13690.15098060191521484No Hit
CCTTAAACCTGTCTTGTAACCTTGATACTTACTTTCAGCCTCCATGTGTC13160.1451354800003088No Hit
ATCTAAGCTTGTCCATTTTTAGGCGGCATTTCAAGTGGCCAGAGAACAGC12860.14182692042583367No Hit
TGGCACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGA12820.14138577914923697No Hit
AGTCTGAAGCGAGAGCTAACAACAGTATCAACAGGTTTAATTTGGATTTG12400.13675379574497182No Hit
GTATATGACTTTTTGGCTCGTCGAGCTTCCCAAAAGCCAGGCAATGGCAA12070.13311438021304917No Hit
ATGTTGTCAGTGCGTGGCACGCACTGACAACATCGTGCCACGCACTGACA11010.12142413638323707No Hit
GTCATAGGAGTTGGGGACAAGAAAATCACCCACCACTGGCATAGGAGTGG10880.11999042723429786No Hit
GAGTTATGAGTGCCCTATGCTGGATGAGGGAGTGGAACCAGATGATGTCG10600.11690243829812107No Hit
TTCATATCCTGTATCATTGACAATCATCCCGCTATGCTGGGAGCCATGCA10290.11348359340449678No Hit
ACGATGTTGTCAGTGCGTGGCACGATGTTGTCAGTGCGTGGCACGATGTT10050.11083674574491667No Hit
TCCTTGTAGAGTGAGAAGGGAGCGTCACGGCTCTTCTAGATCGCCGTGCC9840.10852075404278408No Hit
TCCGTACACAACCCAAGTTGATGTCGTGTTGCACCAGCAATCGACATCAT9830.10841046872363493No Hit
GAATATGACACGCCCTTCAATCTAAGCTTGTCCATTTTTAGGCGGCATTT9770.1077487568087399No Hit
ACTCTATGTCAACTGTTGGCTTGTCCTGTGCCATCACGGTAACGCAGCCT9580.10565333574490564No Hit
TCTTAGAACACGTAAACTTGGCACATGTCACCAAGCTCCCTTTGCCAAAA9550.10532247978745812No Hit
GTACAGATCATGGACCTCGGGCACATGTGTGACGCCACCATGAGTTATGA9310.10267563212787803No Hit
GTGCGTGGCACGGCCATTGCTCGAATTGCCAACCAGGCCAAAGCAAATCC9150.1009110670214913No Hit
CTCAATATGTCTGCAAAAGAACATTAGTGGACAGAGGTTGGGGAAACGGT9140.10080078170234213No Hit
TCGTGCCACGCACTGACAACATCGTGCCACGCACTGACAACATCGTGCCA9130.10069049638319295No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCT2950.063.454644
GAGTCTG3050.061.3741575
GCGACAG954.838512E-1060.6421051
CGACAGT1051.1768861E-954.8666651
AGTCTGA4000.054.009371
TGTTGCC400.006219795453.9974565
AAGCGAG4050.053.3227
GTCTGAA4150.052.0543562
GAAGCGA4200.051.426156
TGAAGCG5600.038.569615
TATGCAA1803.4197E-936.004262
TCTGAAG6100.035.4101223
CTGAAGC6100.035.408174
TTCGAGT5450.034.352692
ACAGTTC1708.315328E-833.8807564
CACGGCC5400.031.9931989
CCTTGGA1204.2227053E-430.0035512
TCGAGTC6250.029.9522423
AGGTTCG200.00628301928.80245685-89
ATGCAAG2253.0122465E-828.800233
Produced by FastQC (version 0.11.2)