FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3036_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3036_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences169163
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT4000.2364583271755644No Hit
GGCTGCTGCCTGTGGTGAAGGCTCATCAGGGAGGAGATAGGGCGAAGGGA3420.2021718697351076No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2100.12414062176717132No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA2020.11941145522366002No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1940.11468228868014874No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA4650.085.207851
AGCAAAA6300.061.729752
CAAAAGC6450.060.2941784
GCAAAAG6600.058.923863
CTAGTGC553.333417E-452.330329
CTACTAG605.0982984E-448.012036
CAAGGTA450.0098823748.012034
GGTTATA450.0098823748.012032
GAGCGAA2150.046.9093551
AAAAGCA8550.046.3273965
AGCGAAA2200.045.829672
AAAGCAG10900.044.928696
ACTAGTG657.5967994E-444.2794958
AACAAGG3500.041.1531682
CGAAAGC2400.039.0097734
GCGAAAG2600.038.778953
AAACAAG3850.037.4230461
GTAATAG800.00209640536.0196841
GCTTTCG800.00209640536.0196841
AAGCAGG14650.033.89977
Produced by FastQC (version 0.11.2)