FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3036_d1_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3036_d1_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences252349
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA10730.42520477592540484No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG10100.40023935105746405No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG6910.2738271203769383No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA6130.2429175467309163No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT4460.17673935700161283No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT4250.16841754871229922No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC3280.12997871994737448No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT3130.12403457116929333No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT2960.11729786922080135No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG2950.11690159263559594No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA2920.11571276287997971No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG2750.10897606093148773No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC2710.10739095459066611No Hit
CCCTTGGCCTCGATATCGAAACAGCCACTCTTGTTGGGAAACAAATCGTG2690.10659840142025528No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT2630.10422074190902283No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA900.0111.960211
GCAGGTA2450.091.051639
GCAGGGT4100.086.001179
GGATAGC554.6140485E-878.5025
AGGTACT406.904509E-571.960164
CAAGGTA300.001992258571.960154
GCAAAAG9100.071.169393
GCGAAAG1700.067.740632
TAGCAAA651.4678153E-766.424768
GATAGCA651.4678153E-766.424766
AGCGAAA1750.065.805191
CCTACTA554.23663E-665.418335
AAGCAGG11800.064.032357
CAGGTAC451.2370486E-463.964593
CACCTAC451.2370486E-463.964593
CGAAAGC1750.061.6801383
AGCAGGG7700.060.745598
GAGCAAA11100.058.3576431
AAAGCAG13300.057.3517046
GTAGAAA1700.055.0392651
Produced by FastQC (version 0.11.2)