FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3035_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3035_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33235
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA1750.5265533323303746No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1610.4844290657439446No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG700.21062133293214985No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC650.19557695200842487No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG640.19256807582367985No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT520.15646156160673988No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA520.15646156160673988No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT470.1414171806830149No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG440.1323905521287799No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC400.12035504738979991No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA400.12035504738979991No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC370.11132841883556492No Hit
GCCGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGG360.10831954265081992No Hit
GGTCCAATCGCACGCAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGC350.10531066646607493No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA253.785135E-8144.01
CAGCTCG100.0072078314144.09
GCGAAAG301.6318036E-596.000013
TACTGTA259.5658394E-486.3999942
ATGACCG259.5658394E-486.3999949
CGAAAGC353.506793E-582.285714
ACTGTAA353.506793E-582.285713
GAGCAAA1350.074.6666641
GCAGGTA300.00197266272.000019
AGCGAAA406.797956E-572.02
GCAAAAG1500.067.2000053
TGTAATG350.003634504761.7142835
AGCAAAA1750.057.6000022
GCAGGGG753.8297367E-757.6000029
AGCAGGG1550.055.7419368
AAGCAGG2350.055.1489337
CTGTAAT400.00616621654.04
TTGCCTC400.00616621654.05
AGCAGGT701.7298453E-551.428578
AAAGCAG2550.050.8235326
Produced by FastQC (version 0.11.2)