FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3034_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3034_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330047
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT3870.11725602717188764No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA3640.11028732271464367No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA10350.089.734691
AGCAAAA12950.071.1517262
GCAAAAG13350.069.559053
CAAAAGC13250.069.540744
GAGCGAA2300.062.60561
AAAAGCA18550.050.0600175
AGCGAAA2900.049.6527181
AAAGCAG21450.048.9971436
GCGAAAG3450.039.6442032
CGAAAGC3350.038.678793
TAGGGCC951.0713872E-437.887125
CGTATAC1001.4488646E-435.9927673
TCGTATA800.002107014935.9927642
AAGCAGG30150.034.6199077
CTATAAT1402.874815E-530.8556181
GTATACC1050.00797339527.4230584
TATACCA2156.4953565E-726.785315
AGCAGGT6100.025.9619968
GCAGGCA7850.025.6763699
GTATATA5100.023.9988141
Produced by FastQC (version 0.11.2)