FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3034_d1_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3034_d1_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences363823
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA14420.3963465751203195No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG13380.36776124654021325No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG10130.2784320947273812No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT5760.15831874290520392No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT5700.15666958933327468No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA5590.15364614111807115No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT5450.149798116116903No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA5330.1464998089730446No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG4890.13440601611223038No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC4410.12121278753679675No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT3960.10884413574732768No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC3850.10582068753212413No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT3830.10527096967481439No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1100.0104.697771
GCAGGTA2750.091.5853659
GTATAGC259.6541987E-486.375671
TTATAGG1650.078.523331
CACCTAC851.8189894E-1276.213823
CCTACTA950.075.7577065
GCAGGGT5250.075.38669
AGCGAAA2200.071.979721
CCACCTA903.6379788E-1271.979712
TCCACCT957.2759576E-1268.191311
GCGAAAG2350.067.385272
GCTATAC554.2335596E-665.436113
CGAAAGC2450.064.634853
GCAAAAG12850.063.297343
AAGCAGG15850.059.937017
TAGCAAA753.9535735E-757.5679478
GAGCAAA14350.056.6808971
GTAGAAA2100.054.841691
AGTAGAA1850.054.4711381
AGCAGGG9650.051.4532178
Produced by FastQC (version 0.11.2)