FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3033_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3033_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences464838
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG7580.16306756332313624No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA6900.14843881094058575No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG6390.13746724665367288No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC5490.11810566261794431No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT5180.11143667256119337No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA14550.079.669751
GAGCGAA3800.071.999671
AGCAAAA18000.063.199722
GCAAAAG18500.062.652443
CAAAAGC19350.059.1434254
AGCGAAA4700.056.6805952
CGAAAGC5000.053.2625584
AAAAGCA23800.048.992365
AAAGCAG29700.047.984296
GCGAAAG5750.045.0732083
GCAGGTA4050.044.4203349
AAGCAGG34350.041.6891677
AGCAGGT8800.036.798218
CGTTACA1251.3259878E-534.5561263
GTACTAT5100.031.0586831
GTTACAC1504.616306E-528.7905734
GCAGGGT8650.028.2852279
GTATGCT3505.456968E-1226.7427391
AGCAGGG27950.025.4888528
CCGTTAC1751.314688E-424.6856042
Produced by FastQC (version 0.11.2)