FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3033_d1_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3033_d1_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences326212
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA13600.4169067968069844No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG11930.3657130945520091No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG9080.27834659669172196No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT6050.1854622147560482No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT5400.16553652226159676No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG4740.14530428065184603No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA4250.13028337400218262No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT4190.12844407931038712No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC3910.11986070408200801No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC3480.10667909212414013No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT3340.10238740450995058No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT3330.10208085539465132No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT3280.10054810981815507No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTATA100.007231645144.034821
GAGCGAA1300.0116.3358151
CGATCCC203.972561E-4107.992995
GCAGGTA3400.091.024999
GCAGGGT5000.080.610049
CCTACTA554.6027708E-878.540355
TCCACCT608.394272E-872.017411
CACCTAC608.4120984E-871.9953163
CCACCTA608.4120984E-871.9953162
AGCGAAA2200.068.722812
CGAAAGC2250.067.195634
GCAAAAG12550.067.119143
TTATAGG1200.066.015961
GCGAAAG2300.065.734863
AAGCAGG15950.062.7323847
GAGCAAA13750.060.7565041
AGCAGGG8500.055.885118
AAAGCAG18900.052.1870886
CAAAAGC16350.050.6389124
AGTAGAA1301.4006218E-1049.8582081
Produced by FastQC (version 0.11.2)