FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3032_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3032_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences439046
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT6940.15806999722124787No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC6310.14372070352537092No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG4920.1120611507678011No Hit
GCCGTTACACCTTTGTTCGAGTCATGATTGGGCCATGAACTTGTCTTGGG4480.10203942183734734No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG4440.10112835557094246No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA14300.081.5692141
GCAAAAG15600.076.600543
AGCAAAA17750.065.707432
CAAAAGC18950.061.9193954
AAAAGCA23650.049.309685
AAAGCAG28350.048.2446176
CGTTACA1700.046.579213
AGCGAAA4250.044.0485571
GAGCGAA4450.042.0688481
AAGCAGG36000.038.1925967
CGAAAGC5450.035.662814
CCAATAT2451.8189894E-1235.262492
GCGAAAG6200.032.5098273
GTTACAC2301.0786607E-931.298284
ATATCAC3101.8189894E-1230.1876985
GCAGGTA3900.029.5327389
GCCGTTA2701.8553692E-1029.3343321
AGCAGGT9150.029.1091128
CCGTTAC2752.3101165E-1028.7976992
TATCCCG1000.006285488628.794425
Produced by FastQC (version 0.11.2)