FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3032_d1_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3032_d1_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences327443
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA12660.38663217720336057No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG11830.3612842540533773No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG8970.2739408080184948No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA5830.17804625537879876No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT5710.17438149540530717No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG5480.16735737212278168No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT4900.1496443655842391No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA4580.13987167232159492No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT4210.12857199573666256No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC4170.12735040907883205No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA3300.10078089927101816No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT3290.10047550260656053No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1350.0133.317761
CACCTAC502.055458E-10100.788243
CCACCTA502.055458E-10100.788242
TCCACCT554.3837645E-1091.625661
CCTACTA554.4019544E-1091.6116645
GCAGGGT4650.089.754829
GCGAAAG2050.087.7946243
AGCGAAA2050.087.7946242
CGAAAGC2100.085.691194
ACAGGTG406.8926456E-571.991591
CAAGGTA702.9685907E-971.980594
GCAGGTA2700.071.958619
GACAGTA406.908318E-571.95867
AGTAGAA1550.065.024671
GCAAAAG11850.061.9674533
AGCAGGG9000.059.9655048
GAGCAAA12250.059.944021
AAGCAGG15800.057.384717
CGATCCC553.331992E-452.3495255
CAATTCG553.3370225E-452.333539
Produced by FastQC (version 0.11.2)