FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3032_d-2_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3032_d-2_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences127118
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT1610.12665397504680692No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA1590.1250806337418776No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAAAG902.9649527E-1064.008742
AGCGAAA954.783942E-1060.6398621
ATAGACC1154.1836756E-1156.333363
TAGACCT1206.366463E-1153.9861374
AGGACGC400.006212700653.986135
GGATATA450.00988121348.0065571
GATAGAG605.1062537E-447.9876757
ATATGTC450.00989659247.987678
GGATAGC450.00989659247.987679
TATAGAC2150.043.5408332
CGAAAGC1351.0802978E-842.655713
GCTATAA700.00109101141.1322943
AGACCTC1651.4442776E-939.2626465
TAGACTC750.001531589138.390144
TGTTATA800.002098669336.004922
GAGCAAA2252.1827873E-1135.204811
ACCTCTT1905.7352736E-934.0965047
ATTAGAG900.003745777531.9917813
CCTCTTA2101.5217665E-830.849228
GCAAAAG2351.3387762E-930.6304263
Produced by FastQC (version 0.11.2)