FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3031_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3031_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80310
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1920.2390735898393724No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA1300.16187274312040842No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA960.1195367949196862No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC910.11331092018428589No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG840.10459469555472545No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATAC100.007234553143.948329
ACCGATA100.007234553143.948328
GTATACC100.007234553143.948324
GAGCGAA502.3479515E-886.4228061
GAGCAAA2350.085.8098751
AGCGAAA608.312418E-872.0190052
GCGAAAG608.348434E-871.974163
AGCAAAA3100.065.049422
CAAAAGC3450.058.413814
GCAAAAG3450.058.413813
CTGTGTA502.0723148E-457.5793279
CCGAATT400.0061916254.014252
GCAGGGT1301.382432E-1049.8282629
AACCGAT450.00988740847.9827737
CGAAAGC901.3756016E-647.9827734
TATGTGT450.00988740847.9827734
AAAGCAG5100.046.5715146
GTATATA951.9877916E-645.4856871
TACAATC657.562536E-444.291797
AGCAGGG3150.043.4129838
Produced by FastQC (version 0.11.2)