FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3031_d1_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3031_d1_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences498736
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG19660.3941965288248693No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA19650.3939960219434731No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG14180.2843187578197684No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT8250.16541817715183985No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG7860.15759840877738923No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT7510.15058066792852332No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA7150.1433624201982612No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA6610.13253504860286805No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT6250.12531680087260594No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC6070.12170767700747491No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT5810.11649449809117449No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT5500.11027878476789323No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT5380.1078727021911392No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT5130.10286003015623497No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC5050.10125597510506561No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA5000.10025344069808474No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1750.0127.538951
GCAGGTA5450.089.805089
GCAGGGT7500.087.331049
TTATAGG1700.076.2329561
AGCGAAA3050.075.538672
CGAAAGC3300.071.9977954
CGATCCC300.001989273471.9977955
GCGAAAG3350.070.92323
GCAAAAG19750.066.347333
AGCAGGG12650.064.294888
AAGCAGG24600.063.796527
GGTACTT350.003664995361.7123951
GAGCAAA22750.057.5982321
GTAGAAA2600.055.3829151
AAAGCAG29200.053.2586446
CAAGGTA953.311652E-853.0510064
TGCCATG3250.050.9369548
CAAAAGC25950.050.773014
CCTACTA1457.2759576E-1249.653655
AGCAAAA26850.049.6074182
Produced by FastQC (version 0.11.2)