FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3031_d-2_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3031_d-2_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences256657
Sequences flagged as poor quality0
Sequence length150
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT6210.24195716462048572No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA5830.22715141219604376No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG4590.17883790428470683No Hit
GGTCTATACTACCCAAAATCTACATATTTGATGTAATACCTATCAAAATA3740.14571977386161297No Hit
GGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCTCGGAGCAGGAA2620.10208176671588931No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAGC2850.085.8719563
GCGAAAG2900.081.925272
AGCGAAA3100.078.962181
CTTATGC750.001533099238.3972971
AATACTA1406.9651287E-735.990455
TAGACCT3100.034.8294684
CCTACAT850.002836383433.8733633
GAGCGAA1501.1929715E-633.5976331
GAAAGCA7550.032.415244
GAGCAAA2900.032.273591
TATAGAC3450.031.3021432
ATAGATA1153.291411E-431.2960474
CATACAT4150.031.2145528
TACATAC3950.030.9791226
GTACAGG1453.6504796E-529.7910061
ATAGACC3400.029.6391943
AAAGCAG10700.029.5996235
CCTCTTA3950.029.1511428
GAATAGA1255.358085E-428.7979741
CATATAC1000.00628288128.7923623
Produced by FastQC (version 0.11.2)