FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3030_d3_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3030_d3_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences298336
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA18550.6217821516679181No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG16370.5487101791268905No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG9420.3157513675855411No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT5830.19541724766705998No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT5730.19206532232114126No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG5220.1749705030569559No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC4870.1632387643462405No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA4530.15184221817011692No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT4480.15016625549715756No Hit
GGGTTTCGACTTTCTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGT4100.13742893918266652No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT4060.13608816904429905No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC4020.13474739890593157No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG3930.13173066609460474No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT3830.12837874074868605No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC3700.12402123779899174No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC3390.11363026922664378No Hit
AGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC3340.11195430655368444No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG3320.1112839214845007No Hit
GAGCGAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG3290.11027834388072509No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA3280.10994315134613322No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT3250.1089375737423576No Hit
GGCTAGCACTACGGCAAAGGCTATGGAACAGATGGCTGGATCGAGTGAAC3240.10860238120776575No Hit
CTCATGGAATGGCTAAAGACAAGACCAATCTTGTCACCTCTGACTAAGGG3070.10290410811970396No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA3040.10189853051592836No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT3020.10122814544674462No Hit
AGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGC3010.10089295291215274No Hit
CTCTAGCTCTATGTTGACAAAATGACCATCGTCAACATCCACAGCACTCT3000.10055776037756087No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA1550.0120.7661741
GCAGGTA3950.091.117929
GACAGTA259.650657E-486.379787
TTACCTG259.650657E-486.379783
GCAGGGT6700.078.4294059
AGCGAAA2450.076.403092
CACCTAC950.075.771743
GCAAAAG13650.074.8835753
GAGCAAA13650.073.8412551
GATTACC300.001988769371.9952241
CCACCTA1000.071.9952242
AAGCAGG17900.068.766037
CCTACTA1050.068.555395
CGAAAGC2800.066.84154
GCGAAAG2800.066.84153
GTAGAAA1950.066.457131
AGCAAAA15800.063.7932362
AGCAGGG10850.061.036418
CGACTAA2650.059.75969
ACGACTA2650.059.75968
Produced by FastQC (version 0.11.2)