FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3030_d1_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3030_d1_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences325223
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA13080.4021855772808196No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG12240.3763571457123266No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG8770.2696611248281948No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA5720.1758793197283095No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG5360.16480999191324106No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT5260.16173517863127762No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT4860.1494359255034238No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC4440.1365217097191773No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA4270.1312945271398394No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT4090.12575986323230523No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT3930.1208401619811637No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC3650.11223068479166602No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA3510.10792594619691719No Hit
TCCTTAGACAGAATCCAACTGAGGAACAAGCCGTAGACATATGCAAGGCA3310.10177631963299029No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTG100.0072373752143.996615
GAGCGAA950.0128.85891
TAGATAC203.969466E-4108.014071
GATACTT203.971896E-4107.997463
GCAGGTA3300.085.0627449
TTATAGG1150.081.401921
CGATCCC451.2829041E-679.998125
GCAGGGT4550.077.5127949
TCCACCT755.1077222E-967.208761
CACCTAC755.114998E-967.198423
CCACCTA755.114998E-967.198422
GTAGAAA2800.066.865861
AGCGAAA2050.063.2277571
GTGAATA350.003661512261.7223321
GCAAAAG11950.061.454623
TGCCATG2300.059.4585728
CGAAAGC2200.058.9077073
CCTACTA1007.6215656E-1057.598655
GCGAAAG2350.055.147642
GAGCAAA13600.054.5365221
Produced by FastQC (version 0.11.2)