FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3030_d-2_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3030_d-2_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224606
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACCTCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT3550.15805454885443845No Hit
GGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCTCGGAGCAGGAA2980.13267677622147228No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA2880.12822453540867118No Hit
GGTCTATACTACCCAAAATCTACATATTTGATGTAATACCTATCAAAATA2630.11709393337666849No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG2540.1130869166451475No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA3900.083.074611
GGAGCGA651.4617581E-766.4596941
GAGCGAA1150.062.6069531
AGCGAAA2250.060.7983061
TACACCG400.006218729553.9864775
CGAAAGC2700.050.6539733
GCGAAAG2950.046.371592
GCAAAAG6400.046.113453
CAAAAGC6500.045.4040074
AGCAAAA7000.044.227342
TATAGAC2501.8189894E-1234.5590362
AAAAGCA9250.031.9055215
GGGCTAT1153.2855317E-431.3034761
ATAGACC2301.0732037E-931.2965093
CAGCTCG1652.5121863E-630.5378049
GCTATAC950.00488803930.3081933
TATACAG2151.9272193E-830.1319855
AAAGCAG12700.028.9061436
GGCTATA1255.3546455E-428.7991982
TATGTTC1050.00796998827.42175
Produced by FastQC (version 0.11.2)