FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3029_d-2_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3029_d-2_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences481467
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT6920.14372739980102478No Hit
GGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCTCGGAGCAGGAA5830.12108825734681714No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA5390.11194952094328375No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGAAA4300.095.438351
CGAAAGC4750.086.387843
GCGAAAG5000.082.068462
GAGCGAA1600.058.497851
CAATTCG450.00991275947.9882629
TTACGCA700.001093983741.1327974
GAGCAAA8800.036.816831
GCAAAAG8650.033.2901153
GTACGTA1102.5388462E-432.7192739
GTCCGGT900.00375586231.9921747
CAAAAGC9050.031.0200394
CTAGGCT1402.8813727E-530.8495984
CCTATTG950.004890908530.3115233
GTATAGT1204.2236282E-429.9988981
AGCAAAA10250.028.795952
GTTACGC1000.006284450628.7959483
TACGCAG1000.00628764128.792965
AGTACGT1255.370452E-428.7929578
TCCGGTC1306.755152E-427.6855358
GAAAGCA14900.027.536894
Produced by FastQC (version 0.11.2)