FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3028_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3028_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145957
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG3740.25623985146310213No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA3490.23911151914604986No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC2550.17470898963393328No Hit
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT2380.16306172365833774No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2170.1486739245120138No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC1910.13086045890227943No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA1840.1260645258535048No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC1540.10551052707304207No Hit
GGCTGCTGCCTGTGGTGAAGGCTCATCAGGGAGGAGATAGGGCGAAGGGA1530.10482539378035996No Hit
GGCAAGTGCACCAGTTGAATAGCTTAGTGACACCTCCTTGGCCCCATGGA1460.10002946073158533No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGTA203.9685523E-4107.989976
GAGCAAA4500.083.1922761
GAGCGAA1350.074.659741
AGCGAAA1350.074.659742
GCAAAAG5600.066.850933
CGAAAGC1650.065.448464
CAAAAGC6100.062.551574
AGCAAAA5950.061.7085532
GCGAAAG1750.061.7085533
GTCTAGA350.003660703361.7085531
GTAGAAA1750.053.4807471
AAAGCAG9400.052.080276
TAGAAAC1800.051.995172
AAAAGCA8050.048.293655
TACTGGG1850.046.6983643
TAACGAC952.004017E-645.4694633
AAGCAGG11250.045.435787
ATCGCAC1704.1836756E-1142.3490077
TCGCACG1704.1836756E-1142.3199928
CCAATCG1903.6379788E-1241.680344
Produced by FastQC (version 0.11.2)