FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3026_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3026_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences512453
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG7450.14537918599364233No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG6650.1297679982359358No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC6510.12703604037833716No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA6450.12586520129650916No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA6310.1231332434389105No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA13950.077.921751
GAGCGAA3500.063.7601931
GCAAAAG18450.058.526273
AGCAAAA18400.058.294082
CAAAAGC20450.052.802434
AGCGAAA4250.052.5083922
GCGAAAG4850.046.0125123
CGAAAGC5050.044.1902354
AAAGCAG30600.043.051246
AAAAGCA27050.040.1851585
AAGCAGG40700.032.7183157
CTATACT3050.030.6831174
CGTTACA1453.6601617E-529.7878553
AGCAGGT10700.029.5993758
GCAGGTA4500.027.1925549
GCCTTAT1701.0812325E-425.4072881
GCAGGGT9400.024.503939
AGCAGGG27950.023.9505088
CATACAT5850.023.3782148
ATAGACC3153.08155E-822.8531153
Produced by FastQC (version 0.11.2)