FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3025_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3025_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1059270
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT13110.12376447931122378No Hit
GATACTGGGAAGGGAGACACGTCAGATCCCCAGAGAGGTTTAGGGGGGAC11730.11073663938372653No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA33050.085.4023361
CAAAAGC41000.068.4752044
AGCAAAA41600.067.324072
GCAAAAG44050.064.551123
AGCGAAA10250.059.710431
CGAAAGC11150.054.8777923
GAGCGAA7900.054.6863981
GCGAAAG11450.054.0763172
AAAAGCA53600.052.7813345
AAAGCAG68900.047.3294646
AAGCAGG84150.039.005187
AGCAGGG64050.026.4095088
TATAGAC9450.023.6180482
GCAGGGG37900.022.9804089
CCGTTAC2552.8760714E-622.587282
GCAGGCA23700.022.171049
GCCGTTA2654.0122377E-621.7369841
GAAAGCA30000.021.1161164
GGCGGTT2053.8492854E-421.0673057
GATACTG11700.020.3087521
Produced by FastQC (version 0.11.2)