FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3024_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3024_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences159241
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT2860.17960198692547774No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA2520.15825070176650485No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG2500.15699474381597703No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG2340.1469470802117545No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA2310.1450631432859628No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA1790.11240823657223957No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG1780.11178025759697564No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC1750.10989632067118393No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA4200.083.995051
GTCTAAA300.00198771.9957662
CGTTACA651.4593388E-766.457633
GAGCGAA1200.065.996121
AGCAAAA5500.062.832662
CTATAGG502.0754621E-457.596611
GCAAAAG6200.056.8998763
AGCGAAA1450.054.617472
CGAAAGC1550.051.0937654
GTTACAC859.314863E-750.8205344
TACCTAT901.3813769E-647.9971737
CAAAAGC7650.046.114934
GCTTTCG803.876972E-544.997351
CTACACC657.573864E-444.3050841
AAAGCAG10150.042.5590676
TTACACC1053.9907536E-641.1404345
GCGAAAG1953.6379788E-1240.612993
TTTCGCT907.767081E-539.9976463
TCGCTCC907.767081E-539.9976465
CAAGGTA750.001531262438.397744