FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3022_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3022_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences88450
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT1620.1831543244771057No Hit
GTATATACATACATATATACTTACACAGTTTGAGCATTCACAGGCCACTA1460.16506500847936686No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG1440.16280384397964953No Hit
GAGCAAAAGCAGGTGCATAAAGATCTGGGTCCTTCTGACCTGGCTATTCA1240.14019219898247598No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA1220.13793103448275862No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC1100.12436404748445451No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG1020.11531938948558507No Hit
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG950.10740531373657433No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG940.10627473148671567No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCGA100.007246465143.877331
TGCTACC100.007246465143.877332
GAGCGAA502.055458E-10100.7141341
GAGCAAA2950.090.228161
AGCAAAA3200.085.427162
GCAAAAG3500.080.1602253
GCGAAAG801.03682396E-1071.938673
CAAAAGC3950.069.206824
CGAAAGC851.7826096E-1067.706984
AGCGAAA755.0913513E-967.142752
AAAAGCA4750.057.5509345
AAAGCAG6000.055.1529776
CAGTTTA553.328253E-452.319039
TCGGGGC450.00990999847.959117
GTAGAAA901.3818953E-647.959112
CAGCTCG605.112762E-447.959119
AAGCAGG7250.046.63617
TAGAAAC1101.0440999E-745.7791523
TAAGGTC657.585128E-444.2699474
TATACAG907.775338E-539.9659275