FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_3021_d8_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_3021_d8_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145144
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGAAGTGGGAGAGGGAAAAGGAGAAGGAAGCTTCCCT5190.35757592459901894No Hit
GGCTGCTGCCTGTGGTGAAGGCTCATCAGGGAGGAGATAGGGCGAAGGGA4290.2955685388304029No Hit
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT2200.1515736096566169No Hit
GCTTCTGTCTTTGGCTCAGGTCATGATCCCAGGATCCTGGGATCAAGTCC1930.13297139392603208No Hit
TGGAGGAGGGCAAGGGTTAGGTCCAAGTGGAATAGTGGCAGGGTGAGTCC1830.12608168439618586No Hit
CATGGAGGAGGGCAAGGGTTAGGTCCAAGTGGAATAGTGGCAGGGTGAGT1810.12470374249021661No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA1620.1116132943835088No Hit
CCCACTTCCCCTGCTTTTGCTCCCCCCAGGGGACCCGGACAGGTCCAGCC1530.10541255580664718No Hit
CCCACTTCCCCTGCTTTCGCTCCCCCCAGGGGACCCGGACAGGTCCAGCC1530.10541255580664718No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCAAA4400.080.161851
CAAGGCG300.001988141871.982078
GCAAAAG5350.064.582053
CAAAAGC5650.063.7009474
AGCAAAA5850.060.292672
GAGCGAA1750.057.585651
AGCGAAA2050.052.6698071
GCGAAAG2250.047.9880452
AAAGCAG9250.047.4692546
CGAAAGC2300.046.9448283
TAGAAAC1402.8921932E-1046.274192
AAAAGCA7750.044.582445
GTAGAAA1803.3887773E-935.991031
AAGCAGG12450.035.2683227
GCAGGGG6300.034.2771769
GTACTAT1102.5302186E-432.7191241
TCTATAT900.003747362931.992031
GTCTTAT950.004882355730.3082371
AGCAGGT2701.8371793E-1029.3260278
TTAGTCG200.0062898528.79283125-129
Produced by FastQC (version 0.11.2)