FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_2042_d3_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_2042_d3_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences301032
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA14230.47270722049483116No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG12550.41689920008504083No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG9460.31425230540274784No Hit
CTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGG6360.21127322012277766No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT4450.1478248159664089No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA4270.14184538520821707No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT4070.1352015732546706No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA4050.13453719205931597No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG4010.13320842966860666No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC3860.1282255707034468No Hit
GTATGCTAGAGTCCCGTTTTCGTTTCATTACCAACACTACGTCCCCTTGC3830.12722899891041484No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT3800.12623242711738286No Hit
TCCACCTACTAGTGCTGACCAACAAAGTCTCTATCAGAATGCAGATGCAT3780.12556804592202822No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT3690.12257833054293231No Hit
GTACTATGTTCTTTTCCATGATCGCCTGGTCCAATCGCACGCAAAGAGGG3520.11693109038241781No Hit
GAGCAAAAGCAGGAGTTTAAAATGAATCCAAACCAAAAGATAATAACCAT3420.11360918440564459No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT3420.11360918440564459No Hit
GGGTTTCGACTTTCTCTTACTTGATCCATCATTGCCCTCTGGGCAGCTGT3350.11128385022190332No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC3200.10630099125674347No Hit
GCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAGCT3170.1053044194637115No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT3160.10497222886603419No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG3090.1026468946822929No Hit
ATTTAAAGATGAGTCTTCTAACCGAGGTCGAAACGTACGTTCTTTCTATC3070.10198251348693826No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCCC100.0072392486143.982385
GAGCGAA1250.0132.485811
GCAGGTA4050.092.417789
GCAGGGT5200.089.974059
GCAAAAG13050.078.335253
TTATAGG1050.075.431881
CGAAAGC2450.073.46044
ACAGGTG300.001987916972.003161
GACAGTA406.8976144E-571.979237
AGCAGGG8350.070.6861958
GCGAAAG2550.070.5796053
AGCGAAA2400.069.003032
AAGCAGG17950.066.565767
CAATTCG651.4660873E-766.4423759
GAGCAAA15650.065.331941
CACCTAC808.521965E-962.992293
AGTAGAA1300.060.9257471
AAAGCAG19950.059.9024476
CCACCTA851.3738827E-859.2967152
CCTACTA851.3757017E-859.2868655
Produced by FastQC (version 0.11.2)