FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_2042_d1_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_2042_d1_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257434
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA12770.4960494728746009No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG10540.4094253284336956No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG8290.3220242858363697No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT4760.1849017612281206No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG4720.18334796491527924No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA4660.18101727044601723No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT4650.1806288213678069No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA3570.13867632092109045No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC3450.13401493198256642No Hit
GACTGAATAAGAGAGGCTATCTAATAAGAGCACTGACATTAAATACGATG3290.127799746731201No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT3160.12274990871446662No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT2900.11265023268099786No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT2650.10293900572573941No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCTTGG100.0072385934143.982711
TGATAGG203.9724703E-4107.987043
GAGCGAA700.0102.844791
GCAGGTA3750.097.889239
TATTCAC259.644776E-486.3896262
GCAGGGT3600.081.974229
CGAAAGC1700.080.460933
AGCGAAA1700.080.460931
AGTAGAA1050.075.419521
GCGAAAG2000.068.3917852
GAGCAAA11650.067.974671
GCAAAAG11700.067.684183
CGATCCC451.234446E-463.992315
AAGCAGG13950.061.928057
GATTACC350.003664279461.7068751
GATAGCA702.443794E-761.7068756
CAAGGTA607.0889437E-659.9811449
AGCAGGG6150.059.688558
AGCAAAA13400.059.097382
TCTACAC1500.057.5930823
Produced by FastQC (version 0.11.2)