FastQCFastQC Report
Thu 22 Feb 2018
HVHNVAFXX_n02_2041_d1_nw.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVHNVAFXX_n02_2041_d1_nw.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306279
Sequences flagged as poor quality0
Sequence length150
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA13250.43261209550769064No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG11180.3650266587000741No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG8700.28405473440882334No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT6590.2151632988223156No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT5650.18447232751837378No Hit
TTATAGGTGCCATGCTCAGGATGTTTCTGAACCACTCGGGTTGATTTCTG4720.15410785590915474No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT3950.12896737941550024No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA3670.11982538796326227No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT3270.10676540017435085No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC3250.10611240078490526No Hit
GTAGATGGATGGTGAATGCCCCATAGCACGAGGACTTCTTTCCCTTTATC3200.10447990231129134No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC3110.10154140505878627No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGAA850.0143.989061
GACAGTA203.9802553E-4107.938867
GCAGGTA3150.093.661239
TTATAGG1450.089.372511
GCAGGGT4250.077.885319
TGCCATG2150.073.632718
CACCTAC502.4071287E-671.959243
CAGGGAA300.001992766571.959246
AGCGAAA2000.068.39481
CCTACTA554.239111E-665.4174965
CGAAAGC2200.062.146623
GCAAAAG13250.061.9121063
GCGAAAG2250.060.795382
AAGCAGG16300.058.2737467
AGTAGAA1007.6215656E-1057.5956231
GAGCAAA14100.057.187141
AGCAGGG7450.056.021968
GTAGAAA1550.055.73771
CCACCTA651.1380387E-555.38042
AAAGCAG18000.051.570796
Produced by FastQC (version 0.11.2)